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Subategory:
Mutants
Description:
R40-9D was recovered as a photosynthesis-competent revertant of rbcL-V331A mt+ (45-3B). It results from an intragenic-suppressor mutation that causes a T342I substitution (ACT-ATT) in the Rubisco large subunit, and the double-mutant enzyme has decreases in Rubisco CO2/O2 specificity and holoenzyme stability. The V331A/T342I mutant-enzyme x-ray crystal structure has been solved. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.