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Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcLΔ-25B1 mt+ (MUT-4700), a V262L substitution (GTA-TTA) was created in the Rubisco large subunit. This is the intragenic-suppressor substitution that complements the L290F substitution in photosynthesis-competent revertant R96-4C (rbcL-L290F/V262L). The V262L substitution alone improves the thermal stability of wild-type Rubisco. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.