Product Name
Lactate Colorimetric/Fluorometric Assay Kit
Product Overview
The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001-10 mM of various Lactate samples.
Description
Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm).
Applications
The kit can detect 0.001-10 mM of various Lactate samples.
Kit Components
• Lactate Assay Buffer
• Lactate Probe (in DMSO)
• Lactate Enzyme Mix
• L(+)-Lactate Standard (100 nmol/µl)
Materials Required but Not Supplied
96-well clear plate with flat bottom
Multi-well spectrophotometer
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Compatible Sample Types
Culture medium; Fermentation medium; Blood; Cells
Datasheet 