Product Name
Triglyceride Quantification Colorimetric/Fluorometric Kit
Product Overview
The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
Description
Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
Applications
The kit can detect 2 pmol-10nmol (or 2-10000μM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
Kit Components
• Triglyceride Assay Buffer
• TriglycerideProbe (in DMSO, anhydrous)
• Lipase
• TriglycerideEnzyme Mix (lyophilized)
• Triglyceride Standard (1 mM)
Materials Required but Not Supplied
96-well white plate (fluorometric) or clear plate (colorimetric)
Multi-well spectrophotometer (Absorbance/Fluorescence reader)
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Compatible Sample Types
Animal tissues: Liver, pancreas, heart etc.
Cell culture: adipocytes etc.
Biological fluids: serum, plasma etc..
Datasheet 