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Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.


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Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

Cat#
AgK-406
Product Name
Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)
Product Overview
Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells.
Description
Autophagy or autophagocytosis (“self-eating”) refers to a process of degradation of cytoplasmic components within lysosomes, a unique process mediated by autophagosomes. Autophagy consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. Autophagosomes engulf a portion of cytoplasm, thus autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases.
Applications
Measurement of Autophagy processes Screening compounds affecting Autophagy
Storage
Store at -20°C.
Size
50 assays
Kit Components
• Assay Buffer (10X) • Autophagy Stain (500X) • Cell Death Marker (500X) • Positive Control Reagent (10000X)
Materials Required but Not Supplied
96-well clear plate with flat bottom Multi-well spectrophotometer
Detection method
Flow Cytometry (FL1/FL2)
Compatible Sample Types
Serum, plasma, blood, urine & other body fluids
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