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Plant ABA Detection Kit

Plant ABA Detection Kit is designed for the detection of hormone ABA in plant tissues and cereal grains. The assay uses a ELISA-based protocol.


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Plant ABA Detection Kit

Cat#
PHD-100
Product Name
Plant ABA Detection Kit
Product Overview
Plant ABA Detection Kit is designed for the detection of hormone ABA in plant tissues and cereal grains. The assay uses a ELISA-based protocol.
Storage
reaction wells: are very sensitive and must be stored at-20ºC (stable for more than 6 months); tracer: reconstituted is stable for 2 days at 2-4ºC. Undiluted stock must be stored at -20 ºC (stable for more than 6 months); substrate: store at 2-4ºC. Working solution is stable for 8 hours at 2-4 ºC; standards: store at -20 ºC (stable for more than 6 months). At 2-4ºC stable for 7 days.
Kit Components
reaction wells: 5pcs for 480 assays, 60 strips with 8 wells tracer: 20 – 50 µl tracer diluent: 5x stock + 0.02 % NaN3 reaction and wash solution: 10xTBS stock+0.02 % NaN3 stopping reagent: 2x stock 5N KOH substrate diluent: 10x NaHCO3 stock +0 0.02 % NaN3 substrate: 100 mg standards: 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol
Scientific Background
This ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The ABA (abscisic acid C1) hormone and ABA-glucosylester specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples.
Detection method
ELISA Detection
Sensitivity
0.01 to 10 pmol/50 µl or 4 pg/50 µl
Sample Type
Plant Tissues
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