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Plant N6-isopentenyladenosine Detection Kit

Plant N6-isopentenyladenosine Detection Kit is designed for the detection of hormone N6-isopentenyladenosine in plant tissues. The assay uses a ELISA-based protocol.


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Plant N6-isopentenyladenosine Detection Kit

Cat#
PHD-104
Product Name
Plant N6-isopentenyladenosine Detection Kit
Product Overview
Plant N6-isopentenyladenosine Detection Kit is designed for the detection of hormone N6-isopentenyladenosine in plant tissues. The assay uses a ELISA-based protocol.
Storage
Reaction wells: very sensitive and must be stored at -20 ° C (stable for more than 6 months) Tracer: store at -20 ° C (stable for more than 6 months). Diluted tracer is stable for 7 days at +4 °C. Standards: store at -20 ° C (stable for more than 6 months). stable for 7 days at +4 ° C. Substrate: store at +4 ° C. Working solution is stable for 5 hours at +4 ° C
Kit Components
Reaction wells antibody coated and blocked: 5pcs for 480 assays, 60 strips with 8 wells. Tracer: 20 – 50 μl alkaline phosphatase conjugate. Tracer diluent: 5x 250 mM TBS Tris, 10 mM NaCl, 1 mM MgCl2, pH 7.5 stock + 0.02 % NaN3. Reaction and wash stock solution: 10x TBS stock+0.02 % NaN3. Stopping reagent: 2x 5 N KOH stock. Substrate diluent: 10x 500 mM NaHCO3 stock, pH 9.6+ 0.02 % NaN3. Substrate: 100 mg p-nitrophenylphosphate. Standards: 600 μl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol and 15.2 fmol.
Scientific Background
This ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The N6-isopentenyladenosine specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples.
Detection method
ELISA Detection
Sensitivity
0.01 to 10 pmol/50 µl
Sample Type
Plant Tissues
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