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Plant Trans-zeatin riboside Detection Kit

Plant Trans-zeatin riboside Detection Kit is designed for the detection of Trans-zeatin riboside in plant tissues. The assay uses a ELISA-based protocol.


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Plant Trans-zeatin riboside Detection Kit

Cat#
PHD-105
Product Name
Plant Trans-zeatin riboside Detection Kit
Product Overview
Plant Trans-zeatin riboside Detection Kit is designed for the detection of Trans-zeatin riboside in plant tissues. The assay uses a ELISA-based protocol.
Storage
Reaction wells: very sensitive and must be stored at -20°C (stable for more than 6 months). Tracer: store at -20°C (stable for more than 6 months). Diluted tracer is stable for 7 days at +4°C. Standards: store at -20°C (stable for more than 6 months); stable for 7 days at +4°C. Substrate: can be stored at +4°C or -20°C. Working solution is stable for 5 hours at +4°C.
Kit Components
Reaction Wells 5 pcs antibody coated and blocked: 480 assays, 60 strips with 8 wells. Tracer: alkaline phosphatase, 20 – 50 El. Tracer Diluent: 5 x concentrated stock: 250 mM TBS Tris, 10 mM NaCl, 1mM MgCl2, pH 7.5 stock + 0.02 % NaN3. Reaction and Wash Solution: 10 x concentrated stock TBS + 0.02 % NaN3. Stopping Reagent: 2 x concentrated stock: 5 N KOH. Substrate Diluent: 10 x concentrated stock: 500 mM NaHCO3 stock, pH 9.6+ 0.02 % NaN3. Substrate: 100 mg of p-nitrophenylphosphate. Standards: 600 µl of each, 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488 fmol, 244 fmol, 122 fmol, 61 fmol, 30,5 fmol, 15,2 fmol, concentration/50 µl. Your samples: plant or algae extract volume 50-250 µl.
Scientific Background
This ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The trans-zeatin riboside specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples.
Detection method
ELISA Detection
Sensitivity
0.01 to 10 pmol/50 µl
Sample Type
Plant Tissues
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