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EHA105 Competent Cell


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EHA105 Competent Cell

Cat#
ACC-103
Product Name
EHA105 Competent Cell
Product Overview
The genotype of EHA105 Competent Cell is C58 (rif r) Ti pEHA105 (pTiBo542DT-DNA) Succinamopine. EHA105 strain was transformed from EHA101 strain, and the background of the EHA105 strain is C58 type. The nuclear gene of EHA105 contains a screening tag - rifampicin resistance gene (rif). In order to facilitate the transformation, the EHA105 strain carries a amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pEHA105 (pTiBo542DT-DNA) plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly.
Applications
EHA105 Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.
Storage
Store at - 80 centigrade for 12 months.
Kit Components
EHA105: 100μl/tube * 10 tube/50 tube/100 tube; pCAMBIA2301(control vector,10ng/μl): 10μl.
Sensitivity
The conversion efficiency of pCAMBIA2301 plasmid in EHA105 Competent Cell is >10^4 cfu/μg DNA.
Sample Type
Rice, tobacco and other plants
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