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AGL1 Competent Cell


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AGL1 Competent Cell

Cat#
ACC-105
Product Name
AGL1 Competent Cell
Product Overview
The genotype of AGL1 Competent Cell is C58 RecA (rif R/carbR) Ti pTiBo542DT-DNA Succinamopine. The background of the AGL1 strain is C58, RecA-type. The nuclear gene of AGL1 contains screening tags - rifampicin resistance gene (rif) and the carbenicillin resistance gene (carb). In order to facilitate the transformation, the AGL1 strain carries a amber basic Ti plasmid pTiBo542DT-DNA without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pTiBo542DT-DNA plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly.
Applications
AGL1 Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.
Notes
1. The volume of the added plasmid should not be greater than 1/10 of the competent volume; When the plasmid is impure or contaminated with organic substances such as ethanol, the conversion efficiency drops sharply. 2. Gently handle when mixing in the plasmid. If a high concentration of plasmid is converted, the amount of bacteria ultimately used for plating can be reduced accordingly. 3. When the density of positive clones on the plate is too large, the positive clones grow slower and the colonies become smaller due to insufficient nutrition. In order to obtain large colonies, the amount of plasmid should be reduced. 4. The concentration of rifampicin should not be higher than 25 μg/ml. The excessive concentration of rifampicin is not conducive to the growth of Agrobacterium and will reduce its growth rate and transformation efficiency. The plate used in the calculation of the conversion efficiency of AGL1 Competent Cell only contains 50 μg/ml kan. If the plate contains 20 μg/ml rif, the conversion efficiency is reduced to 1/2. 5. The purpose of adding rifampicin to the culture medium is to prevent the growth of bacteria and to screen Agrobacterium. According to the resistance of the strain used, the addition of antibiotics used for Ti plasmid screening can prevent the loss of Ti plasmid, but these antibiotics is not conducive to the transgenic operation of Agrobacterium. Therefore, in general, Agrobacterium is not added with these antibiotics, and the probability of loss of Ti plasmid is extremely low (can be ignored).
Storage
Store at - 80 centigrade for 12 months.
Kit Components
AGL1: 100μl/tube * 10 tube/50 tube/100 tube; pCAMBIA2301(control vector,10ng/μl): 10μl.
Sensitivity
The conversion efficiency of pCAMBIA2301 plasmid in AGL1 Competent Cell is >10^4 cfu/μg DNA.
Sample Type
Rice, Arabidopsis, poplar and other plants
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