Ar.Qual Chemically Competent Cell

Ar.Qual Chemically Competent Cell

Size Price Qty Online Order
10 tubes (100μL/tube) $490 Order Online  Add to Quote
20 tubes (100μL/tube) $618 Order Online  Add to Quote
50 tubes (100μL/tube) $938 Order Online  Add to Quote
100 tubes (100μL/tube) $1,448 Order Online  Add to Quote

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Cat#
ACC-117
Product Name
Ar.Qual Chemically Competent Cell
Product Overview
The genotype of Ar.Qual Chemically Competent Cell is Agrobacterium rhizogenes (str R,Cam R) Ar Qual Ri (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The Ar.Qual Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid with a broad host range (corn, tobacco, tomato, citrus, etc.) and streptomycin and chloramphenicol resistance.
Applications
Ar.Qual Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.
Notes
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Storage
Store at - 80 °C for 12 months.
Kit Components
Ar.Qual: 100 μL/tube * 10 tube/50 tube/100 tube.
Transformation efficiency
Transformation efficiency of Ar.Qual Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.
Our products/services are For Research Use Only. Not For Clinical Use!
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