Gene Knock-in Services in Cyanobacteria

Due to the biosynthetic and ecological potential of cyanobacteria, gene functional study about it has attracted much interest from researchers all over the world. Many studies indicate that cyanobacteria can express introduced exogenous gene stably.

Lifeasible provides our global customers gene knock-in services, assisting constructing new stains based on gene functional studies for further applications. Our experienced scientists are willing to assist you with all their heart.

Gene Functional Study in Cyanobacteria

Cyanobacteria are one of the most ancient organisms on Earth, and they have been playing the most important role in biochemical cycles, including those of carbon, nitrogen and oxygen. Due to these features, cyanobacteria have attracted much interest in studying the mechanisms and employing the new findings in applications.

Due to the rapid development of molecular process of genetic, systematic and synthetic engineering, the biorefinery and biosynthesis have been developed with higher rate, higher titer and better productivity as well.

Typical Stains

We provide gene knock-out services in all cyanobacteria strains, including but not limited as follows.

• Synechocystis sp. PCC6803: The most widely utilized cyanobacteria chassis. It is widely used for the studies of core photosynthetic complexes due to its capacity to be grown under photoautotrophic, mixotrophic, or heterotrophic conditions.
• Synechococcus elongatus PCC 7942: The first reported strain to be transformed through natural DNA uptake pathways. It's been extensively used as a model of the circadian clock.
• Nostoc sp. PCC 7120: A filamentous freshwater cyanobacterial strain, and it's used extensively as a model to investigate cellular differentiation.
• Synechococcus sp. PCC7002: A commonly used and fast-growing marine cyanobacterium which display better tolerances to high temperature and strong illuminations.
• Synechococcus elongatus UTEX 2973 and Synechococcus elongatus PCC 11801: Strains with faster growth rate but close-related to slow-growing S. elongatus PCC 7942. Can be used to understand the basic metabolism and to study the mechanism behind.

Our Services

Cyanobacteria can express introduced exogenous gene stably. For those genes which have been functional studied, researchers have huge requirement introducing them into cyanobacteria cells and examine the efficiency of the biosynthesis, biodegradation and so on.

Including model organisms above, we provide knock in services in organisms as follows as well. If the strain you require are not in the list, please contact us and get customized service.

Information Needed from Customers

• Information of strain.
• Information of target gene.

Delivery Results

• Experimental report, including materials, methods, parameters, results, quality control report.
• Cyanobacteria strains of interests.

Project Cycle

• Single gene knock-out: 4-6 weeks.
• Multiple gene knock-outs: According to the number of target genes.

Service Process

Advantages

Lifeasible offers our customers innovative, efficient, customer-tailored and high-quality gene editing services. And we promise to deliver the results and products on time and with high quality. We serve researchers all over the world with heart and soul. Please feel free to contact us.