ATAC Sequencing Service

ATAC-seq (Assay for transposase-accessible chromatin with high-throughput sequencing) is an epigenetics research technique that uses high-throughput sequencing to analyze chromatin regions accessible to Tn5 transposase. It identifies genome-wide open chromatin regions at specific spatiotemporal points, uncovers potential transcription factor binding sites at these open sites, and combines gene expression data to discover key transcription factors.

Lifeasible specializes in the field of insect research. Our ATAC-Seq assays require fewer cells, enabling you to study genome-wide chromatin changes. We also provide guidance on which epigenetic modifications or transcription factors to investigate and which methods to adopt in subsequent experiments.

What Can We Offer?

We offer ATAC-seq services for insect samples, utilizing highly efficient transposases to cleave and mark open chromatin regions across insect genomes at specific developmental stages, tissue types, or environmental conditions. This approach yields genome-wide regulatory sequence information for actively transcribed regions under these spatiotemporal conditions. Our comprehensive insect ATAC-seq service includes both experimental and bioinformatics analysis.

Main technical workflow

• Sample preparation. Isolate nuclei from insect cells at different developmental stages or from distinct tissues.
• Transposition reaction. Tn5 transposase cuts open chromatin regions and inserts sequencing adapters.
• Library construction and sequencing. Amplify insertion fragments and perform high-throughput sequencing.
• Data analysis. Includes quality control, alignment, peak identification, transcription factor binding site prediction, nucleosome positioning, and regulatory element annotation.

Information analysis services

• Sequencing data quality assessment. Filter out low-quality data to ensure data integrity.
• Reference sequence alignment analysis. Distribution statistics of uniquely mapped reads.
• Peak calling. Localize open regions, nucleosomes, and transcription factor binding sites.
• Peak annotation. Investigate chromatin accessibility and identify target genes potentially regulated by transcription factors or other regulatory factors.
• Differential peak analysis. Analyze peaks differing across samples to explore chromatin-open regions specific to particular states.
• Associated gene functional analysis. Perform GO and KEGG enrichment analysis for genes associated with peaks/differential peaks.
• Motif analysis. Investigate transcription factors potentially binding to chromatin-open regions.

Application Areas of ATAC Sequencing

• Developmental biology. Can be used to analyze chromatin dynamics during embryonic development and identify key regulatory factors,
• Behavioral genetics. Used to study chromatin states of genes related to the nervous system and explore behavioral regulatory mechanisms,
• Environmental adaptation and evolutionary studies. Compares chromatin accessibility among different insect populations or species to reveal the epigenetic basis of adaptive evolution,
• Research on drug resistance mechanisms. Analyzes chromatin differences between resistant and susceptible insects to identify regulatory elements governing resistance genes.
• Vector insect studies. Investigates chromatin changes in mosquitoes and other disease vectors during pathogen infection or transmission to inform epidemic control strategies.

Our Service Process

Fig.2 Our service workflow. (Lifeasible)

Why Choose Us?

• Specific shipping protocol. For ATAC-seq studies involving fresh samples, we provide a dedicated shipping protocol to ensure sample viability upon arrival at the laboratory.
• Rapid delivery of experimental results. Our advanced technical platform and streamlined service workflow guarantee the fastest turnaround time for experimental results.
• Comprehensive analysis offerings. We provide standard ATAC-seq analysis alongside multiple advanced analysis options, including ATAC-seq-transcriptome integration studies.

Lifeasible's ATAC-seq service provides crucial support for deciphering epigenetic regulatory mechanisms in insect research. Detecting chromatin accessibility regions reveals the active states of gene regulatory elements, holding significant implications for insect development, physiological regulation, and behavioral mechanisms. If you are interested, please feel free to contact us.