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Chromatin immunoprecipitation sequencing combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to efficiently detect DNA segments interacting with histone modifications, transcription factors, and other elements across the entire genome. By precisely mapping sequencing results to the genome, this technology provides genome-wide insights into DNA segments interacting with histones, transcription factors, and other elements. Lifeasible specializes in the field of insect research. Based on the needs of researchers in insect studies, we provide chromatin immunoprecipitation sequencing (ChIP-seq) services tailored for insect samples. This service is used to analyze protein-DNA interactions, particularly in gene regulation and epigenetic modifications.
ChIP-seq principle
First, chromatin immunoprecipitation (ChIP) technology is used to specifically enrich DNA fragments bound to the target protein. These fragments are then purified and used for library construction. Subsequently, the enriched DNA fragments undergo high-throughput sequencing. The millions of resulting sequence tags are precisely mapped onto the genome, thereby revealing genome-wide information about DNA segments interacting with histones, transcription factors, and other molecules.
| Sequencing | We perform quantitative testing on ChIP samples provided by clients. Upon passing quality control, we proceed with sequencing library preparation, DNA cluster generation, amplification, and high-throughput sequencing. |
| Basic data analysis and output statistics | Sequencing results undergo base calling, with contamination and adapter sequences removed. Statistical outputs include read length, read count, and data yield. |
| Advanced data analysis | ChIP-Seq sequence alignment against reference sequences. Peak calling, statistical analysis of sample peak information (peak detection and counting, average peak length, median peak length). Motif analysis, identifies protein binding sequence preferences. Distribution and coverage depth of uniquely mapped reads across genes and intergenic regions. Providing a list of genes associated with peaks and their GO functional annotations for each sample. Differential analysis of peak-associated genes across multiple samples |
Fig.2 Our experimental process. (Lifeasible)
Lifeasible is dedicated to providing advanced technical support and comprehensive solutions for insect research. Our CHIP sequencing services are meticulously designed at every stage—from material selection and library preparation to sequencing and data analysis—to ensure high-quality research outcomes. If you are interested, please feel free to contact us.
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