Insect Nanopore Full-Length Transcriptome Sequencing

Insect Nanopore full-length transcriptome sequencing is an advanced method that utilizes third-generation sequencing technology to study insect gene expression and transcript structure. This technology reads the sequence of individual nucleic acid molecules using nanopore proteins, enabling the direct acquisition of complete transcripts without the need for assembly.

Lifeasible specializes in the field of insect research, providing advanced technical support for research and development in this area. Our insect Nanopore full-length transcriptome sequencing service enables full-length transcriptome sequencing of insect samples, without interruption, and can directly read the full-length sequence of high-quality individual RNA molecules from the 5' end to the 3' end polyA tail. It can accurately identify alternative splicing (AS), alternative polyadenylation (APA), fusion genes, long non-coding RNAs (lncRNA), and their target genes that cannot be accurately identified by second-generation sequencing, while simultaneously performing quantitative analysis of genes and transcripts.

What Can We Offer?

The Nanopore full-length transcriptome sequencing we provide offers a powerful tool for insect transcriptome research, providing more comprehensive and accurate transcript information to help researchers gain a deeper understanding of insect gene expression regulation and transcript structural variation.

Core experimental services

Sample processing and library construction	Support for insect sample diversity. We support the processing of insect samples at different developmental stages, from different tissues, and under different experimental conditions. RNA quality control. Ensure sample quality. Library construction. Use cDNA-PCR library construction, support Direct RNA/cDNA library construction, and directly obtain full-length transcripts.
Nanopore full-length transcript library construction and sequencing	Utilizing Nanopore long-read technology to obtain complete transcript structures.

Bioinformatics analysis services

Data quality control and preprocessing	Performing raw data filtering, full-length identification, and quality assessment, etc.
Transcript structure analysis	Alternative splicing identification. Precisely identify five types of splicing, including exon skipping and intron retention. Fusion gene detection. Directly identify gene fusion events based on long reads. Alternative polyadenylation (APA). Analyze the regulatory mechanisms of the 3' untranslated region. New transcript discovery. Supplement the insect genome annotation and discover new genes or isoforms.
Quantitative and functional analysis	Transcript quantification. Quantify gene or transcript expression based on full-length sequences. Differential expression analysis. Screen for differentially expressed genes or transcripts in combination with biological replicates. Functional annotation GO enrichment. KEGG pathway enrichment. Transcription factor prediction. lncRNA and non-coding RNA analysis.
Integrated analysis	Species without reference genomes. De novo construction of gene model sets as a reference for RNA-Seq quantification. Species with reference genomes. Correction of gene models and optimization of annotation. Multi-omics integration. Combining genomic and metabolomic data to analyze insect-specific pathways.

Our Technical Advantages

Full-length information. Accurate identification of transcript structural variations such as alternative splicing, gene fusion, and variable splicing.
High-quality data. Compared with second-generation sequencing, our technology service provides higher-quality transcript sequence information and reduces splicing errors.
High throughput. Our technology enables the sequencing of a large number of transcripts, improving research efficiency.
No reference genome required. Suitable for non-model insect species, enabling direct acquisition of complete transcripts.

Application Areas of Our Services

Alternative splicing and isoform studies. Revealing alternative splicing patterns in different tissues, developmental stages, or environmental conditions of insects helps elucidate complex gene regulatory mechanisms.
Functional gene and pathway research. Precisely identifying key genes and their transcript variants associated with insect development, immunity, metabolism, pheromone synthesis, and resistance, providing support for functional genomics.
Long non-coding RNA and regulatory network research. Used to comprehensively identify non-coding transcripts such as lncRNA and circRNA, and to elucidate their regulatory roles in development, behavior, and environmental adaptation.
Insect-environment interaction and adaptation research. Analyzing the transcriptional response of insects to temperature, parasitism, pesticides, or host plant stress through full-length transcriptome analysis to explore environmental adaptation mechanisms.

Our Testing Process

Sample preparation. Extract high-quality RNA.
Library construction. Use the cDNA-PCR library construction method.
Sequencing execution. Perform real-time sequencing using the Nanopore platform.
Data analysis. Includes quality control, transcript annotation, structural variant identification, etc.

Process of testing. Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

Advanced technology platform. We are equipped with advanced sequencing instruments and have a comprehensive, high-throughput ONT full-length transcriptome platform.
Professional technical team. We rely on a highly educated R&D team and have extensive experience in insect research services, spanning many years, as well as a wealth of experience in insect sequencing projects.
Customized services. We can provide customized solutions tailored to meet customer needs and project characteristics.

Lifeasible is at the forefront of the industry in insect research services, offering a full range of Nanopore services from library construction and sequencing to structural analysis and quantitative validation. We focus on identifying transcript structural variations and exploring functional mechanisms, and address insect-specific issues through multi-omics integration. If you are interested, please feel free to contact us.

For research or industrial raw materials, not for personal medical use!

Services

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