Insects are one of the most abundant animal groups in the world. In-depth research into insects can help researchers explore their evolutionary history and internal structure, which is of great significance to scientific research. Lifeasible specializes in the field of insect research, and we understand the importance and wide-ranging applications of insect DNA extraction in the life sciences. We can customize the best insect DNA extraction technology solutions based on the characteristics and requirements of our clients' projects, ensuring the success of their insect research.
We provide comprehensive technical support for insect DNA extraction and can develop the best insect DNA extraction plan based on insect type, different developmental stages, sample requirements, and other special customer needs. Below are some of the techniques we commonly use. In addition, we can also provide other technical solutions based on customer requirements. Please contact us for specific requirements.
Extraction method | Extraction principle | Applicable scenarios | Technical advantages |
Phenol-chloroform extraction method | This technique uses a mixture of phenol or chloroform to disrupt cell membranes, causing proteins to denature and precipitate, while DNA dissolves in the aqueous phase and is separated by centrifugation. | Genome sequencing, library construction. | High-purity, large-fragment DNA can be obtained. |
CTAB technology method | CTAB binds to DNA under high salt conditions and precipitates polysaccharide/polyphenol impurities, making it suitable for insects rich in chitin or pigments. | Insects rich in polysaccharides/pigments (such as cotton bollworms). | Effectively removes impurities. |
Reagent kit (silica gel membrane) | This technology uses lysis buffer to release DNA, which is then adsorbed onto a silica gel membrane using a high-salt buffer solution. Impurities are removed by washing with ethanol. | Suitable for routine PCR and large sample processing. | Ensures greater standardization and safety. |
Chelex method | Chelex-100 is a chemical chelating resin that binds to polyvalent metal ions, thereby inhibiting the activity of DNA enzymes and lysing cells at high temperatures to release DNA. | Rapid identification with morphological preservation. Suitable for DNA extraction from small samples (such as a single insect or insect appendage). | Extremely simple operation, non-toxic, low cost, and highly adaptable. |
Fig.2 Our experimental process. (Lifeasible)
Lifeasible has many years of service experience in insect DNA extraction. We comprehensively consider core factors such as species characteristics, sample status, and downstream applications in insect DNA extraction to provide our customers with efficient, high-quality, low-cost insect DNA extraction services. If you are interested, please feel free to contact us.