Insect Protein Ligand Screening

Insect protein ligand screening refers to the identification of specific interactions between insect proteins and potential ligands through specific technical platforms and methods, with the aim of discovering functionally significant binding molecules. Lifeasible offers comprehensive insect protein ligand screening services. We integrate cutting-edge proteomics analysis, computer-aided virtual screening, and experimental validation platforms to provide clients in the food, functional nutrition, biological pest control, and research sectors with end-to-end technical services spanning from protein characterization to efficient ligand discovery.

What Can We Offer?

Protein preparation and characterization

Proteins are extracted from various edible insects (such as mealworms, grasshoppers, house crickets, etc.).

Proteomics identification and purity analysis are performed using techniques such as SDS-PAGE and nano-LC-MS/MS. We can assist clients in analyzing the physicochemical properties of proteins, including molecular weight, protein type and functional category, and potential allergens.

Virtuascreenings and bioinformatics predictions

Apply structural biology and molecular docking techniques to conduct virtual ligand screenings targeting insect odor-binding proteins (OBPs), olfactory receptors (OR/Orco), and other target proteins. Utilize databases and platforms to support ligand modeling and structural predictions, and accept user-submitted sequence predictions.

Experimental validation and ligand screenings

Establish in vitro binding experiments. Use SPR, bio-layer interference (BLI), and fluorescence polarization to detect protein-ligand binding characteristics.

If required by the client, we can also conduct high-throughput screening (HTS) platform experiments to rapidly identify potential candidate molecules.

Functional validation and quantitative analysis

Conduct activity validation on screened ligand molecules. If they are bioactive ligands, we can further assess their antioxidant activity, etc.

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Experimental techniques-driven technology

Surface plasmon resonance (SPR) technology. By immobilizing receptor proteins expressed in insect cells, this technique enables real-time monitoring of ligand binding kinetics and determination of dissociation constants. This method is highly sensitive, requires minimal sample volume, and can distinguish between the specificity and affinity of ligand binding.
Fluorescence competitive binding technology. Used to detect the binding capacity of insect odor-binding proteins (OBPs) and chemosensory proteins (CSPs) with ligands. By competitively replacing fluorescently labeled ligands, binding strength is quantified, and this method is widely applied in studies of olfactory-related proteins.
Bioluminescence resonance energy transfer (BRET) technology. A donor is inserted into the receptor protein, and ligands are screened by detecting changes in energy transfer signals, making it suitable for high-throughput screening.

Computational simulation-driven technology

Structure-based virtual screening. Utilizes homology modeling, molecular docking, and molecular dynamics simulations for virtual screening of potential binding molecules.
Ligand-based virtual screening. Uses machine learning algorithms to predict new ligands, leveraging big data-driven screening for ligand discovery.

Application Areas of Insect Protein Ligand Screening

Pest control. Discover new, more effective pesticides by targeting specific insect proteins involved in basic processes such as growth, development, or chemical sensing. For example, in the development of behavioral regulators, target-specific proteins are used to screen for attractants or repellents, thereby achieving green pest control.
Disease vector control. Identifying compounds that can prevent insects from transmitting diseases, potentially leading to new vector control strategies.
Basic research. Gaining a deeper understanding of insect biology, particularly in areas such as olfaction and host-pathogen interactions.

Why Choose Us?

Deep expertise in the field of insects. We have a powerful protein library and insect strains, and can supply insects throughout the year.
High throughput and high accuracy. We provide virtual high-throughput screening, combined with experimental verification, which greatly improves screening speed and hit quality.
Customized support. We flexibly adjust screening libraries, detection strategies, and verification methods according to customer needs.
Traceable data quality. All our instruments are calibrated every two weeks, and raw data is permanently stored in the cloud.

Lifeasible is at the forefront of the industry in insect research. We specialize in insect protein ligand screening services, combining computational and experimental approaches to provide efficient and reliable comprehensive services for agricultural and biological research. If you are interested, please feel free to contact us.

For research or industrial raw materials, not for personal medical use!

Services

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