Insect Gene Expression Analysis Services

Insect gene expression analysis aims to utilize advanced biotechnological methods to reveal which genes are expressed, when and where they are expressed, and to what extent, thereby assisting researchers in understanding insect development, physiology, behavior, and mechanisms of response to external environmental stimuli.

Lifeasible offers systematic, precise, and efficient insect gene expression analysis services to meet the diverse needs of research institutions, agricultural enterprises, and biotechnology companies. Drawing on years of experience in insect expression analysis services accumulated through our work in the field of entomology, we provide customized, end-to-end insect gene expression solutions tailored to different species and experimental designs.

What Can We Offer?

RT-PCR

Synthesize cDNA using mRNA as a template, then amplify the cDNA via PCR to detect the expression level of a specific gene. We extract RNA, reverse transcribe it into cDNA, design specific primers for PCR amplification, and finally analyze the results via electrophoresis or real-time fluorescent quantitative PCR (qRT-PCR).

qRT-PCR

By adding fluorescent dyes or probes to RT-PCR, precise quantification of gene expression levels can be achieved. This method is highly sensitive and suitable for the quantitative analysis of small amounts of target genes.

Microarray

Thousands of oligonucleotide probes are fixed on a chip to detect hybridization signals between RNA samples and probes. Through sample preparation, hybridization, washing, detection, and data analysis, we can simultaneously detect expression changes in tens of thousands of genes.

RNA Sequencing (RNA-Seq)

Using high-throughput sequencing technology to sequence RNA molecules, we can precisely quantify the expression levels of each gene and discover new transcripts and alternative splicing events by comparing the sequencing results with the reference genome sequence. We extract RNA, convert it into a cDNA library, perform sequencing, data analysis, and interpretation, enabling us to effectively detect known and unknown genes, with the characteristics of a wide dynamic range and rich data.

Our Advanced Analysis Platform

• PCR instrument.
• High-throughput sequencing technology platform.
• Bioinformatics analysis technology platform.
• Microarray scanner.

Application Areas of Insect Gene Expression Analysis

• Insect development and differentiation. By analyzing gene expression profiles at different developmental stages, we identify key genes and signaling pathways that regulate development.
• Research on resistance mechanisms. By comparing gene expression differences between resistant and susceptible strains, we identify genes and metabolic pathways associated with pesticide resistance, thereby providing a theoretical basis for insect resistance management and the development of new drugs.
• Insect population monitoring and control. Through molecular markers and expression analysis, we achieve dynamic monitoring and precise control of pest populations.
• Basic and applied research. We promote basic research in insect physiology, ecology, evolutionary biology, and other fields. This research can be used in areas such as transgenic insect development and biological control.

Our Testing Process

• Requirement communication and solution design. We communicate with you in depth about the project details and provide you with a customized experimental solution.
• Sample pre-processing. We provide a complete set of sample collection, storage, and transportation solutions, and test the experimental samples.
• Experiment execution. We strictly follow SOPs and monitor the entire experiment through QC.
• Data analysis and reporting. Our bioinformatics expert team will conduct a detailed analysis of the testing data and provide a visual report.
• Follow-up technical support. Based on the report results, we can provide recommendations for secondary verification or functional verification.

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• Professionalism. Our expert technical team has a multidisciplinary background in insect molecular biology, genomics, and bioinformatics, with extensive project experience and data interpretation capabilities.
• One-stop service. We provide a full range of services from sample collection, extraction, and experimentation to data analysis and report interpretation, maximizing service efficiency.
• Customized. Our services cater to model insects, agricultural pests, and non-model insects, providing tailored solutions for each.
• Quality control. We implement comprehensive quality control measures throughout the entire process, from sample collection to library preparation and pre/post-sequencing, ensuring the accuracy and reliability of the data.

Lifeasible has extensive experience in insect gene function research and expression analysis services. We combine cutting-edge technology platforms with a rigorous quality management system to provide you with efficient, reliable, and personalized insect gene expression analysis solutions. If you are interested, please feel free to contact us.