Reduced Representation Bisulfite Sequencing (RRBS)

Reduced representation bisulfite sequencing (RRBS) is a highly efficient and cost-effective DNA methylation detection technology, particularly suited for studying epigenetic regulatory mechanisms. Lifeasible possesses years of experience in providing insect epigenomic sequencing services. Our RRBS sequencing service holds significant importance in insect research, especially in exploring epigenetic mechanisms, environmental adaptability, and behavioral regulation in insects.

Technical features

• Accuracy. Single-base resolution enables precise analysis of methylation status for each cytosine.
• Cost-effectiveness. High value for money, analyzing DNA methylation in promoter and CpG island regions with minimal sequencing data.
• High cost-effectiveness. RRBS delivers rich methylation information using minimal sequencing data.

What Can We Offer?

Our RRBS technology enables single-base resolution DNA methylation detection at the insect genome level, accurately pinpointing methylation sites. This technology efficiently and rapidly identifies differentially methylated regions (DMRs) across different insect tissues, developmental stages, or treatment conditions. Combined with transcriptomic data and gene functional annotation, it enables in-depth analysis of methylation regulatory mechanisms governing key genes associated with phenotypes or physiological processes. This provides robust technical support for studying insect developmental regulation, epigenetic adaptation, stress resistance mechanisms, and evolutionary traits.

Information analysis

• Sequencing data quality assessment. Filter low-quality data to ensure data integrity.
• Alignment against the reference genome. Analysis of alignment rate and coverage.
• Methylation site calling. Analyze methylation sites.
• Methylation distribution. Distribution of methylation across the genome, chromosomes, functional regions, and elements.
• Differential methylation analysis. Identify DMRs.
• Associated gene analysis. Perform GO and KEGG enrichment analysis on associated genes.
• Multi-sample analysis. Use PCA to analyze methylation variation patterns across multiple samples.

Fig.2 Our experimental process. (Lifeasible)

Application Areas of RRBS

• Epigenetic regulation during developmental stages. RRBS sequencing can be used to analyze DNA methylation changes in insects across different developmental stages, revealing mechanisms of gene expression regulation.
• Behavioral and neural regulation studies. RRBS sequencing can be employed to investigate methylation differences between different roles in social insects, exploring the epigenetic basis underlying behavioral patterns.
• Environmental adaptation and stress response. Analyzing methylation responses in insects under environmental stress reveals how epigenetics facilitates rapid adaptation to environmental changes.
• Exploration of resistance mechanisms. In pest research, RRBS can identify methylation markers associated with resistance, aiding in the development of more effective pest management strategies.

Why Choose Us?

• Efficient and high-quality technical services. We utilize advanced high-throughput sequencing platforms to ensure rapid and stable sequencing data analysis and delivery.
• Professional protocol design. Our expert technical team can provide professional and reliable experimental protocols tailored to the specific needs of your project, ensuring high-quality research results.
• Data analysis. Our professional bioinformatics analysis team provides comprehensive data analysis reports to ensure the reliability of your research results.

Lifeasible leverages an advanced technology platform and a robust team of experts to deliver industry-leading RRBS sequencing services. If you are interested, please feel free to contact us.