Insect Transcriptome Sequencing Services
Transcriptome research serves as the foundation and starting point for studies on gene function and structure. Utilizing next-generation high-throughput sequencing technology enables the comprehensive and efficient capture of the full transcript sequence information of specific cells, tissues, or organs of a particular species under specific conditions. Lifeasible's insect transcriptome sequencing service is a specialized scientific research service tailored for RNA sequencing of insect samples, aimed at elucidating gene expression patterns in insects under specific physiological states or environmental conditions.
What Can We Offer?
| Sample processing and RNA extraction |
- RNA extraction from insect tissues or cells.
- Assess RNA quality to ensure the accuracy of subsequent sequencing.
- We can process a variety of insect types, including species without reference genomes.
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| Library construction and sequencing |
- Construct strand-specific or non-specific RNA libraries.
- Remove rRNA to increase the proportion of effective data.
- Use our advanced technology platform for high-throughput sequencing.
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| Bioinformatics analysis |
- Data quality control. Filter low-quality sequences and assess sequencing quality.
- Sequence alignment. Align to a reference genome or perform de novo assembly.
- Gene expression quantification. Calculate expression metrics such as FPKM/TPM.
- Differential expression analysis. Identify genes that show significant changes under different conditions.
- Functional annotation. Annotate gene functions using databases such as GO and KEGG.
- Visualization analysis. Volcano plots, heatmaps, principal component analysis (PCA), etc.
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| Support for insect-specific research |
- Transcriptome assembly and annotation of non-model insects.
- Research on interaction mechanisms with host plants or pathogens.
- Analysis of biological questions such as developmental stages, sex differences, and environmental adaptation.
- Development of molecular markers (e.g., SSR, SNP) for population genetics research.
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Application Areas of Insect Transcriptome Sequencing
Transcriptome sequencing provides information on the abundance of all RNA transcripts with high accuracy, making it widely applicable in insect research.
- Metabolic pathway research. Transcriptome sequencing helps researchers understand the levels of various substances in insects during different physiological stages and in different tissues, thereby clarifying the metabolic pathways of these substances.
- Discovery of new genes. Transcriptome sequencing can efficiently mine gene sequences from insects without reference genomes, which can be used to identify genes related to development, metabolism, immunity, and other processes.
- Discovery of functional genes. Through insect transcriptome sequencing, a large number of genes related to specific traits, such as drug resistance, insect development, and important functional genes, can be identified.
- Study of gene differential expression. Transcriptome sequencing can be used to identify differentially expressed genes in insects under different conditions, providing an important theoretical basis for determining gene functions and developing control strategies.
- Discovery of analytical markers. Insect transcriptome sequencing provides abundant genetic resources for the development of molecular markers in insects.
Sample Requirements
| Sample type |
Recommended sample quantity |
Transportation method |
Storage method |
| Insect tissue |
No less than 300 mg |
Dry ice transportation |
Long-term storage at -80°C |
| Whole insect samples |
No less than 8 specimens |
Dry ice transportation |
Long-term storage at -80°C |
Our Testing Process
Fig.2 Our experimental process. (Lifeasible)
Why Choose Us?
- Short cycle time. Our advanced technology platform ensures fast, efficient, and high-quality sequencing services for each customer in the shortest possible time.
- Diversified omics platform. We are equipped with advanced instruments, including second-generation sequencing platforms and third-generation sequencing platforms.
- Professional bioinformatics team. We provide lncRNA regulatory mechanism prediction, joint analysis of coding and non-coding RNA, and more.
- Comprehensive data. We can perform both standard and integrated analyses of data obtained from insect transcriptome sequencing.
Lifeasible specializes in insect research. We provide insect transcriptome sequencing services to help researchers gain a deeper understanding of insect life activities. If you are interested, please feel free to contact us.
For research or industrial raw materials, not for personal medical use!
