Insect Dual RNA-Seq Service
Dual RNA-seq is an emerging sequencing technology primarily used to study interactions between two or more organisms. Dual RNA-seq technology is increasingly demonstrating its potential in insect research, particularly in elucidating the molecular mechanisms underlying interactions between insects and microorganisms, plants, or other animals. Lifeasible specializes in the field of insect research, providing advanced technical support and reliable solutions for insect research. The Dual RNA-seq technology we offer enables simultaneous analysis of gene expression in two or more interacting species without the need for sample separation, thereby avoiding the interference associated with traditional methods. This technology plays a crucial role in insect research, as insects often engage in complex interactions with pathogens, symbiotic bacteria, or host plants.
Technical Features of Dual RNA-Seq
- Simultaneous analysis. Gene expression analysis can be performed on multiple crop species simultaneously without the need to separate samples.
- Wide range of applications. It has broad application prospects in fields such as disease resistance mechanism research, agricultural disease research, and species evolution.
- Cost-effective. By constructing a transcriptome library, costs can be reduced and efficiency improved.
- Dynamic changes. It can reveal the dynamic changes in gene expression between mutual crop species and their regulatory relationships.
What Can We Offer?
| Dual RNA-seq technology |
This high-throughput sequencing technology enables us to capture the transcriptomes of insects and their interacting partners simultaneously, without separating the samples, thereby preserving expression information under real interaction conditions. |
| Library construction strategy |
- Insects (eukaryotes) and microorganisms (prokaryotes) interactions. We use rRNA chain-specific library construction.
- Insects and plants' interactions. We may use polyA enrichment.
- Insects and other insects' interactions. We select the appropriate library construction method based on the species type.
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| Data analysis content |
- Differential expression analysis. Identify genes that change significantly during interactions.
- GO/KEGG enrichment analysis. Annotate gene functions and pathways to reveal regulatory mechanisms related to insect immunity, metabolism, behavior, etc.
- Co-expression network analysis. Construct gene regulatory networks between insects and their interaction partners.
- Virulence factor annotation. Identify key pathogenic genes of pathogens or parasitic microorganisms.
- Protein interaction networks. Predict protein interaction relationships between insects and their interaction partners.
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Application Areas
- Insect-pathogen interactions. Revealing the immune response mechanisms of insects when infected with viruses, bacteria, or fungi. This can be applied to the development of pest control strategies.
- Insect-plant interactions. This can be used to analyze the defensive responses of plants and the adaptive mechanisms of insects when insects feed on plants, which can help improve plant resistance to insects or develop biological pesticides.
- Insect-microbe symbiotic systems. Interaction transcriptomics can reveal how symbiotic microbes influence insect nutrition, metabolism, development, or behavior. This aids in understanding insect ecological adaptability and evolutionary mechanisms.
- Insect-parasite relationship research. Interaction transcriptomics can reveal molecular mechanisms such as immune suppression and developmental disruption during parasitism, which can be applied to optimize biological control strategies.
Sample Requirements
| Organization type |
Sample submission recommendations |
| Total RNA |
≥2ug，50 ng/ul |
| Fresh insect tissue |
>500 mg |
Our Testing Process
Fig.2 Our experimental process. (Lifeasible)
Why Choose Us?
- We have a high-level bioinformatics team dedicated to providing you with high-quality personalized data analysis services.
- We utilize high-quality sequencing platforms to ensure high coverage and uniformity of valid data.
- We offer comprehensive after-sales service to address any worries regarding project progress.
Lifeasible offers dual RNA-seq services for insect research and provides high-quality transcriptome analysis reports. We are committed to providing personalized analysis services and tailoring our analysis content to customer needs. If you are interested, please feel free to contact us.
For research or industrial raw materials, not for personal medical use!
