Insect Lipidomics Targeted Analysis

Targeted lipidomics analysis of insects offers a valuable tool for insect ecotoxicology, physiological development, and biomarker research, as it enables the precise analysis of specific lipid molecules. Lifeasible is dedicated to the field of insect services. The targeted lipidomics analysis services we provide are characterized by higher sensitivity, specificity, and accuracy in detecting and analyzing target lipids.

What Can We Offer?

We offer targeted insect lipidomics analysis, focusing on specific lipid molecules and their metabolites in insect samples. Based on a separation, characterization, identification, and quantitative analysis system that is highly stable, reproducible, and sensitive, we provide reliable, fast, and cost-effective targeted insect lipidomics analysis services.

Our Analytical Technology Platform

• Liquid chromatography-tandem mass spectrometry (LC-MS/MS). This technology platform has high sensitivity and is suitable for most types of lipids (especially polar lipids such as phospholipids), and is ideal for analyzing PC, PE, PI, Cer, SM, acylcarnitines, and oxylipins.
• Gas chromatography-mass spectrometry (GC-MS). This technique is suitable for volatile fatty acids and methyl esterified fatty acids (FAME).
• UPLC-HRMS (high-resolution mass spectrometry). Suitable for non-targeted screening or precise structural analysis.

Application Areas

• Ecotoxicology. Explaining the effects of pesticides on insect growth, reproduction, and survival through changes in lipidomics provides a scientific basis for assessing the ecological risks of pesticides.
• Physiological and developmental studies. Investigating changes in lipid profiles at different developmental stages of insects to reveal physiological changes in lipid metabolism during development.
• Biomarker screening. In fields such as insect disease mechanism research, cell development research, and nutrition, targeted lipidomics can be used to screen for lipid biomarkers associated with specific physiological or pathological states.
• Standardization research. Establishing standardized lipidomics analysis methods facilitates comparisons of metabolic pathways across different studies and improves the success rate of data validation.

Our Testing Process

• Experimental design. Clearly define the research objectives, establish control and treatment groups, and determine the sample type.
• Sample collection and pretreatment. Rapidly freeze, inhibit lipase activity, extract lipids, and purify using solid-phase extraction (SPE) if necessary.
• Internal standard addition. Add lipid-specific isotope internal standards for quantitative correction.
• LC-MS/MS analysis. Perform multiple reaction monitoring or parallel reaction monitoring on a high-resolution platform.
• Data quality control. Set QC samples for each batch to monitor retention time, peak area, and ion intensity consistency.
• Data analysis. Use advanced data processing and lipid identification software for peak extraction, calibration, and quantification. Additionally, in lipidomics data analysis, we employ PCA, PLS-DA, and KEGG pathway enrichment for biological interpretation.

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• Professionalism. We have long been dedicated to the field of insect biology and have extensive experience in providing services related to insect research.
• Comprehensive. Our services encompass the targeted analysis of hundreds of lipid molecules, catering to the diverse needs of clients ranging from basic research to applied research.
• Advanced analytical platform. We have established a comprehensive lipidomics analysis technology platform capable of achieving high-sensitivity detection and high-reproducibility analysis of lipids.
• One-stop service. From design, extraction, detection, to analysis, we provide a "one-stop service" to ensure the efficient progression of projects.

Lifeasible is committed to providing reliable technical support and solutions for targeted analysis of insect lipidomics. If you are interested, please feel free to contact us.