RNA Immunoprecipitation Sequencing (RIP-seq)

RNA immunoprecipitation sequencing technology combines chromatin immunoprecipitation (RNA Immunoprecipitation, RIP) with next-generation sequencing (NGS), enabling efficient detection of RNA sequences interacting with specific proteins, such as regulatory proteins and transcription factors, across the entire transcriptome. This technology precisely maps sequencing results to the genome to identify RNA sequences or types interacting with specific proteins across the entire transcriptome. Lifeasible specializes in providing advanced, comprehensive insect biological services. We focus on insect RIP-seq services to assist researchers in studying interactions between RNA and RNA-binding proteins (RBPs) within insect cells, playing a crucial role in revealing gene expression regulatory mechanisms.

What Can We Offer?

Core process of RIP sequencing

• Use specific antibodies to identify and precipitate target RNA-binding proteins and their bound RNA.
• Isolate and purify the RNA.
• Analyze the sequence information of these RNAs through high-throughput sequencing.
• Identify RNA-binding sites, enrichment regions, and their functions using bioinformatics tools.

Our analysis content

• Sequencing data quality assessment.
• Alignment against the reference genome.
• Peak calling.
• Inter-sample correlation analysis.
• Motif analysis.
• Gene annotation associated with peaks.
• Differential peak analysis.
• Functional analysis of associated genes.

Application Areas of RIP-seq

• Revealing the functions of insect RNA-binding proteins. RNA-binding proteins in insects play crucial roles in regulating development, immune responses, and stress responses. RIP-seq can be used to identify the RNA targets of these proteins, thereby revealing their regulatory networks.
• Investigating non-coding RNA-protein interactions. Insects harbor abundant non-coding RNAs, including miRNAs and lncRNAs. RIP-seq aids in mapping interaction networks between these RNAs and RBPs, exploring their roles in developmental or behavioral regulation.
• Researching insect stress resistance mechanisms. RNA-protein interactions change when insects confront stresses like high temperatures, pesticides, or pathogens. RIP-seq can analyze alterations in these interactions under stress conditions, further revealing how insects adapt to environments through post-transcriptional regulatory mechanisms.

Our Testing Process

• Preliminary communication and protocol design.
• Sample and antibody preparation.
• Immunoprecipitation (IP).
• RNA extraction and library preparation.
• Bioinformatics analysis.
• Report delivery and technical support.

Why Choose Us?

• Automated platform. Our automated workstations enable parallel processing of multiple samples daily, significantly reducing turnaround times and enhancing reproducibility.
• Stringent quality control system. We implement rigorous quality control across all experimental aspects to ensure reliable results.
• Complex sample processing capabilities. We handle difficult-to-lyse tissues and insect samples with high metabolic interference.

Fig.2 Our service advantages. (Lifeasible)

Lifeasible offers advanced and reliable insect RIP sequencing services, leveraging core strengths in low-input starting material, single-base resolution, and multi-omics integration. We have established a standardized platform covering dozens of insect species and tissue types. Choose our insect RIP sequencing to unlock rich regulatory insights even from scarce insect tissues, accelerating progress in your insect research projects. If you are interested, please feel free to contact us.