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Insect RNA Extraction Services

Insect RNA Extraction Services

Insect RNA extraction holds significant importance and broad application prospects in fields such as entomological research, pest control, and genetic engineering. Lifeasible specializes in the field of insect research. We rely on advanced technical platforms and an experienced team of experts to provide comprehensive technical support for insect RNA extraction.

The Significance of Insect RNA Extraction

Basic research. Extracting insect RNA can be used to gain a deeper understanding of insect gene expression patterns, gene functions, and gene regulatory networks, providing important evidence for basic research in entomology.

Pest control. By analyzing pest RNA, it is possible to study pest gene expression and regulatory mechanisms, which can help develop new pest control strategies.

What Can We Offer?

We select insect RNA extraction techniques based on the insect species, sample size, throughput requirements, budget, and downstream applications provided by our customers. We aim to provide high-quality insect RNA extraction while maximizing customer project requirements. Below are some commonly used extraction methods. If you would like to try other extraction techniques, please contact us.

Chemical reagent method
Extraction method Extraction principle Applicability
Trizol method Cells are lysed using the strong denaturant guanidine thiocyanate, and RNA is extracted using phenol-chloroform. Suitable for small insects or insects with high chitin content, as it effectively inhibits nucleases and prevents RNA degradation.
SDS-KAc method SDS lyse cell membranes, and KAc precipitates impurities. Optimized for small and medium-sized insects such as aphids, it is low-cost and safe to operate.
The extracted RNA is highly intact and suitable for RT-PCR and cDNA library construction.
Kit method
Extraction method Extraction principle Applicability
Extraction kit (silica membrane method) After RNA is released by the lysis buffer, impurities are removed through purification using an adsorption column. Suitable for insects with low chitin content and soft abdomens containing large amounts of body fluid, such as bees, as it efficiently removes proteins and polysaccharides.
The extracted RNA has high purity and integrity, making it suitable for high-sensitivity experiments.
Silica bead adsorption method Silica beads specifically bind to RNA, rapidly removing contaminants. In small insects, extraction efficiency and quality are high, and the method is simple to operate and cost-effective.

Our Testing Process

  • Online consultation. Customers contact us to inquire about insect RNA extraction services.
  • Sample receipt and registration. Customers send insect samples to our laboratory according to the contract requirements. Upon receipt of the samples, we will conduct a detailed registration of the samples.
  • RNA extraction and quality control. After the samples pass inspection, we will perform RNA extraction following standardized operating procedures. Following RNA extraction, we will use multiple quality control methods to assess the quality of the RNA.
  • Results, feedback, and delivery. We will deliver the RNA products to the customer according to the contract terms. For customers requiring further experiments, we will provide technical support and experimental recommendations.

Process of our service.Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

  • Professional team. We have accumulated rich experience in the field of insect RNA extraction and are skilled in handling various insect samples to ensure high-quality and efficient RNA extraction.
  • Advanced equipment. We have advanced RNA extraction equipment and detection instruments to meet the needs of different customers and ensure the accuracy of RNA extraction.
  • Quality control. We have established a strict quality control system from sample reception, RNA extraction, to final product testing.
  • Customized services. We can handle various types of insect samples and samples from different tissue sites.

Lifeasible focuses on insect research, and we are committed to providing various advanced insect RNA extraction technologies to help customers obtain high-quality RNA and promote research and development in the field of insects. If you are interested, please feel free to contact us.

For research or industrial raw materials, not for personal medical use!
Services
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