Insect RNA extraction holds significant importance and broad application prospects in fields such as entomological research, pest control, and genetic engineering. Lifeasible specializes in the field of insect research. We rely on advanced technical platforms and an experienced team of experts to provide comprehensive technical support for insect RNA extraction.
Basic research. Extracting insect RNA can be used to gain a deeper understanding of insect gene expression patterns, gene functions, and gene regulatory networks, providing important evidence for basic research in entomology.
Pest control. By analyzing pest RNA, it is possible to study pest gene expression and regulatory mechanisms, which can help develop new pest control strategies.
We select insect RNA extraction techniques based on the insect species, sample size, throughput requirements, budget, and downstream applications provided by our customers. We aim to provide high-quality insect RNA extraction while maximizing customer project requirements. Below are some commonly used extraction methods. If you would like to try other extraction techniques, please contact us.
Chemical reagent method | ||
Extraction method | Extraction principle | Applicability |
Trizol method | Cells are lysed using the strong denaturant guanidine thiocyanate, and RNA is extracted using phenol-chloroform. | Suitable for small insects or insects with high chitin content, as it effectively inhibits nucleases and prevents RNA degradation. |
SDS-KAc method | SDS lyse cell membranes, and KAc precipitates impurities. | Optimized for small and medium-sized insects such as aphids, it is low-cost and safe to operate. The extracted RNA is highly intact and suitable for RT-PCR and cDNA library construction. |
Kit method | ||
Extraction method | Extraction principle | Applicability |
Extraction kit (silica membrane method) | After RNA is released by the lysis buffer, impurities are removed through purification using an adsorption column. | Suitable for insects with low chitin content and soft abdomens containing large amounts of body fluid, such as bees, as it efficiently removes proteins and polysaccharides. The extracted RNA has high purity and integrity, making it suitable for high-sensitivity experiments. |
Silica bead adsorption method | Silica beads specifically bind to RNA, rapidly removing contaminants. | In small insects, extraction efficiency and quality are high, and the method is simple to operate and cost-effective. |
Fig.2 Our experimental process. (Lifeasible)
Lifeasible focuses on insect research, and we are committed to providing various advanced insect RNA extraction technologies to help customers obtain high-quality RNA and promote research and development in the field of insects. If you are interested, please feel free to contact us.