Insect Enzyme Activity Testing Services

Insects are rich in various functional enzymes, including proteases, lipases, amylases, detoxifying enzymes, cellulases, and various oxidoreductases. These enzymes play a crucial role in insect digestion, development, defense, and environmental adaptation. With the advancement of biotechnology, the potential of insect enzymes in fields such as biological pesticides, bioconversion, and pharmaceuticals and chemicals is increasingly attracting attention. Lifeasible is a professional insect research and service company. We offer insect enzyme activity testing services to help research institutions and businesses explore insect enzyme resources, accelerate research conversion, and develop products.

What Can We Offer?

We offer a wide range of insect enzyme activity assays, covering metabolic enzymes, detoxification enzymes, immune enzymes, and pheromone-related enzymes. Our commonly used assay methods primarily include colorimetric assays (DNSA, TMB color development), chromatography (HPLC), immunoassays (ELISA), and luminescence assays. When selecting assay methods, we consider sample characteristics and adhere to strict operational protocols to ensure the accuracy of assay results.

We offer targeted testing for enzyme types and functions

• Digestive enzymes. Examples include cellulases (β-1,4-glucanase, β-D-glucosidase), chitinases, and proteases. Enzyme activity is assessed by measuring the production of reducing sugars (DNSA method, TB method) or the degradation products of substrates (e.g., N-acetylglucosamine).
• Detoxification metabolic enzymes. For example, glutathione S-transferases (GSTs), where we assess their catalytic efficiency toward specific substrates.
• Oxidase enzymes. For example, cytochrome P450, where we utilize fluorescence-based methods, such as ethyl coumarin-O-deethylase (ECOD), to detect activity and evaluate pesticide resistance.
• Immune-related enzymes. For example, phenol oxidase (PO), we detect activity by measuring changes in absorbance at 490 nm.
• Pheromone metabolic enzymes. For example, esterases and aldehyde dehydrogenases are detected using highly sensitive luminescence methods.

Our Advanced Testing Platform

• Fully automated liquid workstation for microplates.
• High-precision spectrophotometer, fluorescence or luminescence detector.
• Analytical instruments such as HPLC and LC-MS.

Application Areas of Insect Enzyme Activity Testing

• Antibiotic resistance monitoring. We assess the level of insecticide resistance by quantifying the activity of detoxification enzymes (GSTs, P450) in insect populations.
• Physiological mechanism research. Insect enzyme activity testing can be used to analyze the enzymatic basis of insect digestion, immunity, or pheromone synthesis.
• Biopesticides and pest control. By utilizing inhibitors of specific digestive enzymes or detoxification enzymes, we develop green and efficient insect pesticides, identify key enzymes in target insects, and guide resistance management.
• Industrial enzyme resource development. We identify insect enzymes with high temperature tolerance and strong acid-base adaptability for use in industrial catalytic applications such as textiles, food processing, and wastewater treatment.

Our Testing Process

• Sample shipment. When shipping samples (such as insect tissue or intestinal contents), we will let the customer know beforehand about the required sample conditions.
• Sample evaluation and contract signing. We will provide a quote based on the complexity of the testing project (such as enzyme types, testing methods). After both parties confirm the cooperation, a confidentiality agreement will be signed to ensure data security.
• Experiment execution. Our professional technical team will conduct testing using standardized methods and implement strict quality control throughout the entire experiment.
• Report delivery and interpretation. We will issue authoritative testing reports and provide follow-up technical support and report interpretation.

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• A rich insect resource library. We have hundreds of insect resources collected from fields and bred in laboratories, covering important agricultural pests, model species, and economically important insects.
• A professional technical team. Our team members have backgrounds in insect physiology, biochemistry, and molecular biology, with years of experience in enzymology and high-throughput screening.
• Strict quality management. Our experimental processes adhere to rigorous laboratory management systems to ensure the reliability and reproducibility of our data.
• Flexible customization capabilities. From small-scale validation to pilot-scale scaling, we provide optimal solutions tailored to different scales and budgets.

Lifeasible's insect enzyme activity testing services cover the entire process from sampling to reporting, focusing on the functional testing of multiple types of enzymes. These services leverage various technologies to meet the needs of scientific research, resistance monitoring, and industrial applications. If you are interested, please feel free to contact us.