Insect Ribosome Profiling (Ribo-seq) Service

Ribosome profiling (Ribo-seq) is a high-throughput sequencing technology used to study ribosomal footprinting during translation. By capturing and sequencing mRNA fragments protected by ribosomes, Ribo-seq provides detailed insights into translation efficiency, ribosome occupancy, and the dynamics of protein synthesis. This technology is widely applied to decipher gene expression regulation, identify translation initiation sites, and discover novel protein-coding regions. Lifeasible specializes in the field of insect research. We provide Ribo-seq technical support tailored for insect studies, empowering researchers to advance their work in insect functional genomics, developmental biology, and drug resistance research.

Advantages of Ribo-seq Technology

• High sensitivity and resolution. Ribo-seq detects translational activity across the entire genome at single-base resolution, delivering exceptional sensitivity and precision.
• Transient and quantitative. Ribo-seq captures the translational state instantaneously and quantitatively analyzes translation levels.
• Globality and systemicity. Ribo-seq simultaneously detects translational activity across thousands of genes within a cell, enabling the construction of comprehensive translational maps.

What Can We Offer?

Information analysis

Standard analysis workflow. We provide Ribo-seq single-omics analysis and correlation analysis with mRNA-seq data.

Customized analysis workflow. We offer Ribo-seq correlation analysis with ncRNA-seq and proteomics data.

Ribo-seq single-omics analysis includes gene translation level quantification, translation level differential analysis, and translation-related site analysis, comprising the following components.

• Ribo-seq sequencing analysis
• Quantitative level analysis.
• Sample correlation analysis.
• Differential analysis.
• Functional enrichment analysis of differentially expressed genes.
• Translation site analysis.

Associative analysis

We have established a comprehensive Ribo-seq multi-omics associative analysis workflow to empower researchers in conducting in-depth studies, featuring the following key components:

• Ribo-seq and mRNA associative analysis
• Ribo-seq and ncRNA associative analysis
• Ribo-seq and proteome associative analysis

Application Areas of Ribo-seq

• Utilizing sequencing to study active mRNA translation.
• Investigating transcriptional control and post-transcriptional regulation.
• Predicting protein abundance.
• Identifying translation initiation sites.

Sample Requirements

• Insect tissue. Not less than 400 mg.

Our Service Process

• Sample submission. Guide clients in preparing samples and submitting them to us, we also offer on-site collection.
• Sample quality assessment. Our company evaluates received samples to ensure suitability for Ribo-seq experiments.
• Experimental design. This process primarily involves ribosome isolation—specifically, separating ribosomal complexes from cells using tailored methods.
• RNA extraction and library construction. mRNA fragments bound to ribosomes are extracted from the ribosomal complexes. The extracted mRNA is reverse transcribed into cDNA and assembled into libraries suitable for high-throughput sequencing.
• High-throughput sequencing. We prepare libraries for sequencing, including quantification and dilution. Sequencing is performed using high-throughput sequencing technology, generating vast quantities of short read sequences.
• Data processing and analysis. Raw data quality control, sequence alignment, and translation efficiency analysis.
• Result presentation and reporting. We present analysis results in the form of charts and graphs.

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• Customized services. We provide tailored solutions based on the specific characteristics of our clients' insect research projects.
• Optimized experimental workflow. For precious tissues such as micro-fat bodies, heads, and ovaries, we have refined our library preparation protocols to ensure data quality.
• Extensive experience. To date, we have successfully applied our methods to numerous model and non-model insects.
• Stringent quality control. We implement rigorous quality control measures at every stage of the experimental process to guarantee the reliability of final results.

Lifeasible leverages an advanced technology platform and a team of experienced experts to provide Ribo-seq technical services and solutions for insect research. If you are interested, please feel free to contact us.