Whole Genome Bisulfite Sequencing (WGBS) Service

Whole genome bisulfite sequencing (WGBS), recognized as the gold standard for methylation profiling, enables comprehensive, efficient, and highly accurate methylation studies across the entire genome of species with reference genomes. This approach constructs a genome-wide DNA methylation map with single-base resolution. Lifeasible is committed to providing advanced technical support and comprehensive solutions for research in the field of insect biology. We offer WGBS services tailored for insect samples to help researchers uncover epigenetic regulatory mechanisms governing insect development, behavior, reproduction, and environmental adaptation. This provides critical data support for studies on agricultural pest resistance, ecological conservation strategies, and fundamental research using insect models.

Experimental principle

Bisulfite treatment converts unmethylated C bases in the genome to U. After PCR amplification, these convert to T, distinguishing them from the original methylated C bases. Combined with high-throughput sequencing technology, this enables simultaneous analysis of various methylation patterns and the methylation status of DNA sequences corresponding to mRNA, lncRNA, miRNA, and other gene types. Enables high-precision genome-wide methylation studies in species with reference genomes.

What Can We Offer?

Our service process

• Upon receiving DNA samples, we conduct quality control testing. Only samples that pass quality control undergo library preparation.
• After library preparation is complete, we perform quality control testing on the libraries. Libraries that pass quality control are submitted for sequencing.
• Sequencing results are aligned against the reference genome for subsequent standard information analysis and personalized analysis.
• After obtaining raw sequencing data, we perform data filtering to remove low-quality data.
• Following data filtering, the amount of usable data is assessed. Upon passing this assessment, the usable data is aligned against the reference genome to generate alignment results.
• After confirming the alignment quality meets standards, information analysis is performed to produce both standard and personalized analysis results.

Standard information analysis

• Basic data processing and quality control.
• Genome-wide methylation level analysis.
• Distribution ratios of CG, CHG, and CHH in methylated C bases.
• Methylation level distribution of methylated CG, CHG, and CHH.
• Sequence feature analysis of bases adjacent to methylated CG, CHG, and CHH sites.
• Chromosome-level density distribution of methylated C bases.
• Methylation distribution characteristics across different genomic regions.
• Average DNA methylation levels in distinct transcriptional elements across the genome.
• Detection of genome-wide differentially methylated regions (DMRs).
• GO and pathway analysis of genes associated with DMRs.

Application Areas of Whole Genome Bisulfite Sequencing

• Development and reproduction regulation. Revealing the pivotal role of DNA methylation in processes such as ovarian development and embryo formation.
• Environmental adaptation mechanisms. Aiding understanding of how insects respond to environmental changes like temperature and humidity through epigenetic regulation.
• Life cycle regulation. Identifying methylation differences across developmental stages to decipher molecular mechanisms governing life cycle transitions.
• Gene expression regulation. Uncovering epigenetic mechanisms governing gene expression through methylation profiling analysis.
• Pest control and evolutionary studies. Providing molecular foundations for precision pest management and insect population evolution research.

Our Testing Process

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• High-quality service. Our services feature technical stability and excellent reproducibility, ensuring the reliability of experimental data.
• Stringent quality control. From sample testing to library construction and sequencing, every step follows strict standard operating procedures (SOPs) and corresponding quality control standards.
• Extensive experience. Our expert team possesses years of experience in WGBS sequencing services, accumulating rich expertise in service delivery.

Lifeasible is an insect biotechnology company dedicated to providing advanced technical support and comprehensive solutions for insect research. If you are interested, please feel free to contact us.