Reagent 1: Probe-based qRT-PCR buffer
Reagent 2: Probe-based qRT-PCR enzyme mixture
Reagent 3: Fluorescent PCR template dilution buffer
Reagent 4: Mange mite general Probe-based qRT-PCR primer mixture
Reagent 5: Mange mite general Probe-based qRT-PCR positive control (1×10E8 copies/μL)
Reagent 6: qRT-PCR probe
Features
Ready to use immediately; users only need to provide the sample RNA template.;
The primers are carefully optimized for high specificity, amplifying only Mange mite general and showing no cross-reactivity with other pathogens.
A positive control is provided to easily distinguish false negative samples.
Single-tube quantitative PCR detection avoids subsequent contamination.
This kit is sufficient for 50 probe-based quantitative RT-PCR reactions in 20 μL systems..