Product Name
                            
                            
                                c-Myc tag, Biotin conjugated (mouse monoclonal) (clone 9E10)                            
                         
                                                
                                                
                                                
                            
                                Immunogen
                            
                            
                                Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106                            
                         
                                                
                                                
                            
                                Background
                            
                            
                                c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.                            
                         
                                                
                            
                                Format
                            
                            
                                Liquid at 1 mg/ml.                            
                         
                                                
                                                
                            
                                Purity
                            
                            
                                Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin.                            
                         
                                                
                                                
                            
                                Reactivity
                            
                            
                                c-myc tagged proteins                            
                         
                                                
                            
                                Confirmed reactivity
                            
                            
                                c-Myc tagged fusion proteins                            
                         
                                                
                            
                                Tested applications
                            
                            
                                Immunoprecipitation (IP), Immunohistochemisty (IHC), paraffin,  Flow cytometry (Flowcyt), Western blot (WB)                            
                         
                                                
                            
                                Recommended dilution
                            
                            
                                1-5 µg/ml (Flowcyt)                            
                         
                                                
                            
                                Storage
                            
                            
                                Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.