Detection of Malondialdehyde in Plant Cells

Malondialdehyde (MDA) is a natural lipid oxidation product in living organisms. Lipid oxidation occurs when plant cells are subjected to oxidative stress. Some fatty acids are oxidized and gradually decomposed into a series of complex compounds, including MDA, which is widely used as an indicator of lipid oxidation because lipid oxidation can be detected by measuring the level of MDA. When plant organs age or under adverse conditions, membrane lipid peroxidation often occurs, and MDA is one of its products, which is often used as an indicator of lipid peroxidation, indicating the degree of cell membrane lipid peroxidation and the strength of the plant's response to adverse conditions.

Lifeasible is dedicated to plant research, and we are involved in many areas of plant research. Relying on our advanced technology platform, we can provide researchers with high-quality physical and chemical analyses, cellular composition testing, cellular function analysis, and other technical services in plant cell biology research. So far, we have accomplished many vital projects in the quasi-measurement of plant cell MDC, which plays an important role in helping researchers reveal the physiological state of plant cells and play an essential role in the research of plant cell senescence and stress resistance.

Significance of MDC detection

In plants, free radicals acting on lipids undergo peroxidation, and the product of oxidation is malondialdehyde, which leads to cross-linking and polymerization of proteins, nucleic acids, and other macromolecules of life and is cytotoxic. Oxygen free radicals cause cellular damage through the peroxidation of polyunsaturated fatty acids in biological membranes and the breakdown products of lipid hydroperoxides. The product of lipid oxidation, MDA, affects the mitochondrial respiratory chain complex and the activity of key enzymes in mitochondria in vitro, and its production exacerbates membrane damage. Therefore, the detection of malondialdehyde can reflect the degree of lipid peroxidation in plant organisms and thus indirectly reflect the degree of cell damage.

MDA content is a common indicator for studying the physiology of plant senescence and resistance physiology, and its content can reflect the degree of damage suffered by plants from adversity. The accumulation of MDA may cause certain damage to membranes and cells, and the degree of membrane lipid peroxidation can be known through MDA, thus indirectly determining the degree of damage to the membrane system and the plant's resistance. The level of its content can be used as one of the indicators to examine the severity of the stress to which the cells are subjected. Its main damage is to cause membrane lipid peroxidation, which destroys the structure of biological membranes, mainly cytoplasmic membranes, so that the cell membrane is structurally and functionally damaged, and the permeability of the membrane is altered, thus affecting the normal conduct of a series of physiological and biochemical reactions.

Methods for detecting the MDA in plant cells

We offer several validated assays for determining malondialdehyde (MDA) in plants. In method selection, we choose the most suitable assay according to the needs and characteristics of the client's project. Some of our longest currently available assays are shown below.

• Thiobarbituric acid reactive substance (TBARS) assay. This is one of our most frequently used MDA assays. It involves the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) at high temperatures to form a pink chromogen that can be quantified spectrophotometrically.
• HPLC. HPLC is a highly sensitive and specific technique for quantifying MDA. It involves separating MDA from other compounds in the sample based on their chemical properties and detecting them using ultraviolet light or fluorescence.
• Gas chromatography-mass spectrometry (GC-MS). GC-MS has shown good determination in MDA detection, and we utilize this technique to provide qualitative and quantitative information. Generally, when using GC-MS to detect MDC in plants, we first separate MDA from the sample matrix by gas chromatography and then perform mass spectrometry.
• Fluorometric determination. Fluorometric methods involve the reaction of MDA with a fluorescent reagent to produce a fluorescent product. Fluorescence can be measured using a fluorometer with high sensitivity.
• Enzymatic assays. Enzymatic assays utilize the reaction of MDA with a specific enzyme, such as acetaldehyde dehydrogenase, to produce a measurable product. Enzymatic assays are utilized for high specificity and sensitivity in MDA detection.

What do we offer?

At the end of the project, we will provide a detailed technical report, including the following:

• Experimental procedure
• Relevant parameters
• Pictures.
• Raw data.
• MDC content/activity information.

Our services workflow

Lifeasible is committed to plant life science research; we have a professional plant cell biology research team and comprehensive advanced experimental equipment, a standard management system, and strict operation of the quality control system, aiming to provide customers with accurate and reliable technical services. Please feel free to contact us for more information.