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RNA Pull Down Assay


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RNA Pull Down Assay Inquiry

RNA pull down is a popular RNA-centric approach to study RNA-protein interaction. As a leader in biotechnology, Lifeasible has established a state-of-art platform for RNA-protein interaction research. We offer high-quality RNA pull down assay for customers all over the world.

RNA pull down assay depends on the principle that streptavidin agarose bead has a strong affinity to biotin-labeled RNA, and can effectively and specifically pull down biotinylated RNA–protein complexes. The proteins eluted can be detected by western blotting or mass spectrometry. Lifeasible provides both in vitro and in vivo RNA pull down analysis. For exogenous RNA pull down, the shorter RNAs are practically synthesized chemically while longer ones are commonly transcribed from a DNA template by in vitro transcription. The chemical synthesis or in vitro transcription RNA are labeled with biotin and incubate with nuclear, cytoplasmic, or total protein extracts (Figure 1 left). For endogenous RNA pull down, 20-90nt biotinylated RNA probes are designed according to targeted RNA sequence and then incubate with streptavidin agarose beads and cell extracts (Figure 1 right). Native or cross-linked conditions can be used for endogenous RNA pull-down.

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Figure 1. Workflow of RNA pull down assay (Cipriano and Ballarino, 2018)

Lifeasible offers a full array of services that cover every step of the RNA pull down assay, including:

  • Chemical synthesis or in vitro transcription RNA (exogenous RNA pull down).
  • Preparation of biotin labeled RNA.
  • Preparation of streptavidin agarose beads.
  • Binding biotin labeled RNA onto streptavidin agarose beads.
  • Incubation of cell extracts with the immobilized biotin labeled RNA.
  • Elution of the bound proteins from the streptavidin agarose beads.
  • Analysis of the RNA interacting proteins by western blot or mass spectrometry.

With a wealthy of experience in RNA-protein interaction analysis, we proudly provide tailored RNA pull down service with high sensitivity, reliable quality, and competitive price. Through valuable national and international corporations with renowned companies and institutes, we can provide innovative solutions in RNA pull down assay. We are committed to providing the optimal protocol for each project and ensure the success of your project. Please feel free to contact us. Our customer service representatives are available 24 hours a day.

Reference

  1. Cipriano A.; Ballarino M. The ever-evolving concept of the gene: The use of RNA/protein experimental techniques to understand genome functions. Front. Mol. Biosci. 2018, 5:20.
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