Widely-Targeted Metabolomics for Plants

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Primary metabolism and secondary metabolism are the two types of metabolism in plants. All plants have primary metabolism metabolites, such as sugars, lipids, amino acids, and others, which are required for supporting cellular life processes. The secondary metabolites produced are also a class of small molecule organic compounds that are not essential for plant growth and development, while many secondary metabolites are involved in plant resistance, disease resistance, and other processes or have physiological activity and other roles. This is true even though secondary metabolism in different species, organs, tissues, and growth and development periods of specific species are different. Widely-targeted metabolomics can provide a valuable foundation for analyzing plant metabolic pathways and biological questions by detecting these types of substances.

Lifeasible use LC-MS/MS technology to perform broadly targeted quantitative metabolomic detection and analysis of plant metabolites. The widely targeted metabolomics service we provide for plant metabolites combines non-targeted technology with targeted MRM technology, which has the technical advantages of identifying multiple substances, accurate characterization and quantification, wide dynamic range, high sensitivity, and good reproducibility. Currently, it is widely used in the detection of complex plant metabolites.

The database introduction of widely-targeted metabolomics for plants

Plant widely targeted metabolome database
Substance category	Number	Representative substance
Flavone	3700+	Rutin, Phloretin, Pomiferin, Hesperitin, Liquiritigenin, Pelargonidin 3-O-glucoside, Ginkgo biflavones, Formononetin, Theaflavins
Phenolic acid	2100+	Chlorogenic acid, Momordicoside A, Oleuropein, Salvianolic acid, Veratric acid, Salidroside, Pericin B, Magloside A, Vanillic acid
Alkaloids	7000+	α- Solanine, Peimine, Nuciferine, Stachydrine, Matrine, Camptothecin, ArecolinE, DIMBOA, Oat alkaloid A, Lycorenin
Terpenoids	8000+	Artemisinin, Genipin, Paclitaxel, Wilforlide A, Protopanaxadiol, Saikosaponin A, Cucurbitacin B, Crocin-I, Cyclocarioside I, Ecliptasaponin A
Steroids	1300+	Asparagus saponin C, Polyphyllin D, Timosaponin A-III, Gracillin, Sarsasapogenin, Tigogenin, Digitonin, Oleandrin
Vitamins	50+	Ascorbic acid, Riboflavin, All-trans-Retinol, Vitamin U, Ginkgotoxin, Nicotinic acid, Nicotinamide, Vitamin D3, Tocotrienol
Amino acids	540+	Tryptophan, Theanine, Beauvericin, Dencichine, Heterophyllin, Saccharopine, (S)-3-(Allylsulphinyl)-L-alanine, DOPA, S-Adenosyl-L-methionine, Glutamic acid cysteine
Nucleotides	120+	Adenine, Cytosine, Thymine, Inosine, Eritadenine, Xanthosine, Cordycepin, Sepiapterin, Adenosine 5'-monophosphate
Sugar and alcohol	340+	Glucose, Fructose, Sucrose, Fucose, Xylitol, Rhamnose, Maltose, Raffinose, Allitol, Mannitol
Lipids	500+	Linolenic acid, 4-hydroxysphingosine, Lauric acid, Cerulenin, Myristic acid, Palmitic acid, Arachidonic acid, Stearic acid
......	......	......
Total	30000+

Applied directions

Research on biotic and abiotic plant adversity interactions.
Plant growth and development research.
Nutrition and color metabolism of fruits, vegetables, and flowers.
Crop quality traits and breeding improvement research.
Gene function analysis and metabolic pathway research.
Research on plant function and active ingredients.

Advantages of our technology

Comprehensive testing platform. Non-targeted metabolome and targeted metabolome assay technology for metabolite detection with qualitative and quantitative accuracy.
Comprehensive database. Self-constructed database of exclusive metabolites of plant species with a wide range of substances in the database.
Standard product database. Covering thousands of plant species' primary and secondary metabolites.
Leading mass spectrometry platform. We have an advanced mass spectrometer with sensitivity up to the ng/ml level.
Strict quality control system. We are equipped with SOP guidance documents for the whole process and an internal standard for isotopes and QC quality control.

Sample requirements

Sample type	Various plant tissues and liquid samples.
Sample volume	Fresh 2 g, dry 1 g, lyophilized 250 mg, liquid 500 µL.
Storage and transportation	Store in liquid nitrogen or -80 °C. Adequate amount of dry ice for transportation; avoid repeated freezing and thawing.

Our technology routes

Lifeasible is dedicated to plant research and provides high-quality plant analysis services based on our advanced technology platform. If you are interested in us, please feel free to contact us.

Our products/services are For Research Use Only. Not For Clinical Use!

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