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Plant Hormone Analysis


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Plant Hormone Analysis Inquiry

Plant hormones are highly dynamic chemical messengers that play essential roles in the regulation of plant physiological processes, including cell growth, organ differentiation, and response to biotic and abiotic stresses. Therefore, analysis and monitoring of plant hormones forms a valuable tool in plant performance improvement and new crop trait development.

As the plant hormones often present at fairly low concentrations, and their amounts and distributions and are actively changing, it is critical to analyze the hormone levels in real time with high sensitivity. Lifeasible, as a leading plant biotechnology with a long history of plant physiological studies, provides both biosensor-based and mass spectrometry-based approaches that ensure continuous and accurate detections of plant hormones. 

Biosensor-based methods allow continuous monitoring of hormone levels, as well as visualization of local distributions of targeted hormones. Lifeasible provides a list of biosensors that can be applied to the detection of a wide range of hormones in various types of plant tissues.

  • Electrochemical biosensors. Utilizing current or impedance changes resulted from enzymatic redox reactions as sensory readout; intermediate compounds that are specific to certain plant hormones can also be used as biosensors or electrodes to monitor hormone levels in real-time (Figure 1).  
  • Immunosensors. Single stranded RNAs or DNAs, also known as aptamers, fold to create binding sites for ligand molecules. Specially engineered aptamers can bind to targeted plant hormones.
  • Genetically encoded biosensors. Genetic reporters of plant hormones are designed based on the promoter regions of the targeted plant hormones.  


Figure 1. A schematic illustration of electrochemical biosensor setup for measurement in tomato root exudate (Novak, Napier et al. 2017).

Mass spectrometry-based methods are highly sensitive approaches that allow accurate identification and quantification of plant hormones.

  • Gas chromatography-mass spectrometry (GC-MS). Gas chromatography (GC) is an efficient method for the detection and sorting of volatile organic compounds (VOC). When combined with mass spectrometry (MS), compositions of hormones can be separated and analyzed in a linear format (GC-MS), with high fidelity.
  • Liquid chromatography-mass spectrometry (LC-MS). Similar to GC-MS, the LC-MS technology combines high-throughput compound separation with ultra-sensitive detection methods, allowing the analysis of plant hormone molecules within a complicated biological matrix.  

With state-of-the-art technologies, as well as our teams of excellent scientists and experts, Lifeasible is devoted to providing our worldwide customers with the best services at competitive rates. Our high-capacity lab facilities allow hormone analysis of a wide range of plant species, at cell, tissue and whole plant levels. Moreover, we proudly offer our customers with one-stop services, covering all steps from experimental designing to data reporting. Welcome to contact us for questions, inquiries or collaborations.

Reference

  1. Novak, O., R. Napier and K. Ljung (2017). "Zooming In on Plant Hormone Analysis: Tissue- and Cell-Specific Approaches." Annu Rev Plant Biol 68: 323-348.

Table 1 List of detectable plant hormones and related compounds at Lifeasible

Name Name
Indole acetic acid (IAA) Kinetin (KT)
Methy Indole acetic acid (IAA) Cis-zeatin (cZ)
Indole-3-carboxaldehyde (ICA) Trans-zeatin (tZ)
Indol butyricacid (IBA) Cis-zeatin-riboside (cZR)
Indolepropionic acid (IPA) Trans-zeatin-riboside (tZR)
Gibberellin A1 (GA1) Dihydrozeatin (DZ)
Gibberellin A3 (GA3) Cis-zeatin-9-glucoside (cZ9G)
Gibberellin A4 (GA4) Trans-zeatin-9-glucoside (tZ9G)
Gibberellin A5 (GA5) Cis-zeatin-O-glucoside (cZOG)
Gibberellin A6 (GA6) Trans-zeatin-O-glucoside (tZOG)
Gibberellin A7 (GA7) Trans-zeatin-7-glucoside (tZ7G)
Gibberellin A8 (GA8) N6-Isopentenyladenine (IP)
Gibberellin A13 (GA13) N6-Isopentenyladenine riboside (IPR)
Gibberellin A14 (GA14) Isoprene adenosine (IPA)
Gibberellin A15 (GA15) N6-Isopentenyladenine-9-glucoside (IP9G)
Gibberellin A19 (GA19) N6-Isopentenyladenine-7-glucoside (IP7G)
Gibberellin A20 (GA20) N-(Phenylmethyl)-9H-purin-6-amine (6BA)
Gibberellin A24 (GA24) Brassinolide (BL)
Gibberellin A29 (GA29) 24-epi-brassinolide (2,4-epi-BL)
Gibberellin A44 (GA44) Chestnut sterone (CS)
Gibberellin A51 (GA51) 6-deoxo chestnut sterone CS (6-deoxo CS)
Gibberellin A53 (GA53) Homobrassinolide
Abscisic acid (ABA) 28-Homo-brassinolide (28-homoBL)
Abscisic aldehyde 28-norbrassinolide (28-norBL)
Xanthoxin (XAN) 28-norcastasterone (28-norCS)
Jasmonic acid (JA) Teasterone (TE)
Methyljasmonate (MEJA) 28-norteasterone (28-norTE)
Dihydrojasmonate (H-JA) Typhasterol
Jasmonoyl-L-Isoleucine (JA-LIE) Ethylene (ETH)
Salicylic acid (SA) 1- amino cyclopropane carboxylic acid (ACC)
Methylsalicylate (MESA) 1- amino cyclopropane carboxylic acid synthase (ACCS)
Strigolactone (SL) Naphthylacetic acid (NAA)
5-deoxo-strigol (5-DS) Putrescine
Strigol Spermidine
Orobanchol Spermine
Others  
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