Targeted Metabolomics of Carotenoids

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Carotenoids is an essential class of natural pigments, terpenoids, commonly found in animals, higher plants, fungi, algae, yellow, orange-red, or red pigments. Carotenoids are essential components of photosynthetic organisms. Carotenoids are powerful antioxidants that efficiently quench and scavenge free radicals generated in the body, play an essential role in cell membranes, and interact with other protein molecules.

Lifeasible has established a carotenoid analysis method based on LC-MS/MS external standard + isotope internal standard platform, which can detect multiple carotenoids. The method has low throughput, accurate characterization, and can accomplish absolute quantification of metabolites. We can provide technical service for the whole process, from sample extraction to the presentation of the result report.

What the carotenoids do?

Photosynthesis. Carotenoids have the capacity to absorb light energy and transmit it to chlorophyll molecules, increasing the spectral range that the plant can utilize.
Effects of antioxidants. Plants can fight off oxidative stress and lessen the consequences of oxidative damage on plant cells thanks to carotenoids. Plants' cellular stability and structural integrity depend on carotenoids to function properly.
Immune system management. Carotenoids are essential for plant growth and survival because they aid plants in their defense against infections and the impacts of environmental stress.
Nutritive worth. With advantages including antioxidant, anticancer, and preservation of vision health, carotenoids are vital nutrients and the body's primary supply of vitamin A. Vitamin A is intimately tied to human health.

We offer a database of detectable carotenoids

The carotenoid assay detects 68 carotenoids, including carotenoids and carotenoid esters.

NO.	Compounds	Class	CAS NO.	KEGG ID	Quantification type
1	α-carotene	Carotene	7488-99-5	C05433	Absolute quantification
2	lycopene	Carotene	502-65-8	C05432	Absolute quantification
3	γ-carotene	Carotene	472-93-5	C05435	Absolute quantification
4	β-carotene	Carotene	7235-40-7	C02094	Absolute quantification
5	phytofluene	carotene	27664-65-9	C05414	Absolute quantification
6	ε-carotene	Carotene	472-89-9	C16276	Absolute quantification
7	antheraxanthin dipalmitate	Lutein	-	-	Semi quantification
8	lutein caprate	Lutein	-	-	Semi quantification
9	zeaxanthin	Lutein	144-68-3	C06098	Absolute quantification
10	β-citraurin	Lutein	650-69-1	C20894	Absolute quantification
......	......	......	......	......	......

Applied directions

Molecular mechanism of coloration in fruits and flowers.
Plant anti-aging and antioxidant research.
Carotenoid synthesis regulation research.
Research on plant aroma.
Application of carotenoid targeted metabolism.
Nutritional quality of fruits.

Advantages of our technology

Precise quantification. External standard quantification, standard curve linearity of 0.99 or more.
Wide database. Covering 68 carotenoids, including lycopene, β-carotene, octahydro lycopene, and lutein.
High sensitivity. Based on an advanced mass spectrometry platform to ensure high sensitivity.
Experienced. Thousands of individual project experience covering hundreds of species.
Strict quality control. Check the secondary spectra of sample materials and check standard products one by one.

Sample requirements

Sample type	Various plant tissues
Minimum sample size	600 mg
Minimum sample size at risk	300 mg
Biological repetition	3-6
Conditions of transportation	The samples can be frozen with liquid nitrogen and stored at -80 degrees Celsius or sent by dry ice directly.

Our technology routes

Fig. 2. Our technology route - Lifeasible

Lifeasible has leading technology, first-class R&D and production facilities, and a complete plant-targeted metabolome service system seamlessly connecting upstream and downstream experiments. We also have high-quality after-sales service to help you analyze the final report of your project and reduce your worries. If you are interested in us, please feel free to contact us.

Our products/services are For Research Use Only. Not For Clinical Use!

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