Embryo rescue is an in vitro culture technique used to assist in the development of an immature or weak embryo into a viable plant. The embryo rescue technique plays an important role in modern plant breeding. It has been widely used for producing interspecific and intergeneric hybrids, as well as progenies of incompatible crosses which lead to embryo abortion. Lifeasible is a specialized plant biotechnology company featured in plant tissue culture and plant breeding. We offer tailored protocols of embryo rescue for customers around the world.
Embryo culture is the most commonly used method for embryo rescue. Generally, seeds from controlled pollination plants are collected prior to the expected embryo abortion occurrence, followed by isolation and excision of embryos, which are then placed directly onto culture medium for the generation of fertile plants. Besides embryo culture, ovule and ovary culture, which are more suitable for small seeded species or very young embryos, are also utilized for embryo rescue.
Figure 1. An outline of the embryo rescue process of sorghum (Rizal et al., 2014)
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