Plant Subcellular Organelles Fluorescence Imaging

Plant subcellular organelles include the nucleus, endoplasmic reticulum, Golgi apparatus, chloroplasts, mitochondria, vesicles, centrosomes, and peroxisomes, which are the basic structures that sustain the normal life activities of plants. The plastids and vesicles can be distinguished under the light microscope, while the other organelles are usually observed with an electron microscope. With advances in biotechnology and the demand for higher-quality imaging of plant subcellular organelles, fluorescence imaging techniques have been widely used to image organelles. Changes in organelle structure, function, and interactions can be visualized by fluorescence microscopy and fluorescent dyes.

Lifeasible, as a globally renowned biotechnology company, has always been committed to a human-centered service philosophy. Based on our advanced imaging technology platform, we can provide our customers with high-quality fluorescence imaging services of plant subcellular organelles, which can help them understand organelle positioning, organelle structure, function and interactions in detail, etc.

What We Offer

Organelles play a key role in cellular function and the use of appropriate organelle dyes, probes, or specific antibodies for the detection of specific organelles is critical in cell and tissue fluorescence imaging. Our one-stop shop for plant subcellular organelle fluorescence imaging services will try to meet your needs from designing and synthesizing specific fluorescent probes to analyzing imaging results. Our services include but are not limited to, the following items.

• Design and synthesis of fluorescent materials and probes
• Modification of target organelles
• Controlled spatio-temporal coupling of fluorescent probes to target organelles
• Fluorescence imaging
• Analysis of imaging reports

Our Fluorescence Imaging Facilities

With the rapid development of science and technology, fluorescence microscopy imaging is becoming more and more widely used in the biomedical field. Our imaging technology platform offers the following types of fluorescence microscopes.

• General fluorescence microscopes
• Laser scanning confocal microscopes
• Super-resolution microscopes
• Multiphoton laser scanning microscopes

Fluorescent Dyes

The most common fluorescent materials used for fluorescence imaging include organic fluorescent dye molecules, fluorescent proteins, and fluorescent nanomaterials. These probes are essential for long-term tracking of biological processes, low autofluorescence tissue imaging, and time-resolved fluorescence imaging. We offer optimized fluorescent dyes with low cytotoxicity, high photostability, near-infrared (NIR) emission, two-photon excitation, and long fluorescence lifetimes.

Applications

• Reveal organelle interactions in plant cells.
• Contributes to a better understanding of the function of organelles in plant cells.
• Helps to identify specific proteins in plant cells and the location of targets of interest, etc.

Advantages

• Enables precise and specific labeling of plant organelles.
• The use of SIM, PALM, and STORM imaging technologies enable resolutions down to the nanometer level.
• The different research needs can be met in terms of clarity of imaging, fineness of imaging structure, and depth of imaging.

Deliverables

• Fluorescence imaging images
• Experimental results files
• Graphs for publication
• Description of some of the methods used in the publication

Our Service Process

Lifeasible's fluorescent imaging services label and identify organelles of interest to you in fixed and live cells using specific fluorescent dyes. If you are interested, have questions, or would like to collaborate, please contact us directly.