Vector Building Service

Lifeasible can customize your DNA vectors to meet your experimental needs. We have a wide range of vector systems optimized for various research applications, all validated in vitro and some in vivo. Using our platform for vector customization and cloning will significantly reduce the time and cost of traditional DIY vector construction in the lab and increase the efficiency of vector construction.

Conventional vector building service

Vector construction from standardized backbones and standardized components provided by us. These include shRNA vector building, gRNA vector (single gRNA) building, gRNA vector (double gRNA) building, and expression vector building.

Complex vector building service

Complex vectors use a standardized backbone but are assembled from more complex components that need to be constructed. They are usually constructed in multiple steps and involve complex manipulation processes, such as amplifying large fragments, fusion of multiple fragments, gene synthesis, and targeted mutagenesis. For cloning other types of fragments, such as high GC fragments, simple repeats, or segmented repeat sequences, additional costs and lead times may be required.

Advanced vector building services

• Cloning vector building service
• Viral vector building service
• Expression vector building service
• Shuttle vector building service
• Secretion vector building service
• Report gene vector building service
• CRISPR/Cas9 vector building service

Advantages

• A highly intuitive carrier design platform helps you quickly customize the carriers you need.
• Large selection of stock vector backbones and vector components to help you quickly construct vectors.
• Reliable experimental data are available for vector backbones and vector components.
• Flexible vector cloning strategies for both simple and complex vectors.
• Experience with challenging vector construction projects.
• Rapid access to downstream services such as plasmid DNA extraction and viral packaging.
• Customers own the IP of their customized vectors.

Quality control

Our vectors undergo stringent quality control steps to ensure the sequence is consistent with the design. Commonly used quality control methods include DNA purification, A260/280 assay, enzymatic characterization, Sanger sequencing, plasmid extraction from Glycerolactobacillus, and re-transformation.

Customer-supplied materials

If the customer chooses to provide the materials for vector construction, we will perform restriction endonuclease digestion and characterization of the vector backbone provided by the customer, as well as sequencing of critical regions for verification. These vectors are non-standard vectors, and the price of the vectors depends on the situation.

Delivery

The final delivery form is E. coli, containing the target vector. For different needs, we can provide plasmid DNA extraction services in different sizes, including small extraction (>10 μg), medium extraction (>100 μg), large extraction (>300 μg), extra large extraction (>1 mg), and extra large extraction (>10 mg). For viral vectors, viral packaging is offered as a downstream service in various sizes.

We will provide efficient and professional vector construction service with ultra-low prices and fast cycle time. The price of our cloning service is lower than the cost of reagents you need to prepare clones by yourself, which can save you time and effort. All customized vectors undergo strict quality control to ensure that the delivered vectors meet your design requirements. In addition to molecular cloning of DNA vectors, Lifeasible offers a variety of related services such as plasmid DNA preparation, viral packaging (lentivirus, AAV, adenovirus, MMLV, baculovirus, etc.), library construction, BAC mutagenesis, point mutation, stable transient cell line construction, and RNA preparation. We welcome your inquiries.