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Mutation Breeding


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Lifeasible is a dominant plant biotechnology company. We provide tailored services for mutation breeding in plants. Mutation breeding is a powerful tool for the characterization of plant gene functions, specific biological processes, as well as correlations between genotypic and phenotypic characters, which has been widely applied in crop trait improvement.

Plant mutations can be induced by physical stimulations (e.g., X-rays, α- and β-particles, fast neutrons, and UV light), which often lead to chromosome changes, or by chemical treatments (e.g., ethyl methanesulfonate (EMS)) which typically cause point mutations. Alternatively, mutations can also be introduced through advanced genetic engineering technologies such as CRISPR/CAS9 and RNAi.

Mutations not only can be easily detected by regular polymerase chain reaction (PCR) approaches, but also can be screened by high-throughput technologies such as whole genome sequencing. Targeting induced local lesions in genomes (TILLING) is also a popular method that allows high-throughput detection of random mutagenesis within the genome. TILLING is a highly feasible technology, which has been applied in a variety of plant species including Arabidopsis thaliana, Medicago truncatula, Lotus japonicus, Brassica rapa, tomato,rice, maize, barley, lotus, poplar, and wheat. The most commonly used types of TILLING are:

  • Deletion-TILLING: a TILLING variant based on a neutron-induced mutagenesis system called Deleteagene (Delete-a-gene).
  • High Resolution Melting (HRM) – Based TILLING: a high-throughput method that relies on DNA melting curves derived from fluorescent dyes.
  • Conformation-Sensitive Capillary Electrophoresis (CSCE)-Based TILLING: a method for which detection of mutations is based on heteroduplex analysis of DNAs.
  • Eco-TILLING (ecotype TILLING): an efficient method for detecting SNPs in nature population.
  • DEco-TILLING: a cheaper and easier modification of TILLING and Eco-TILLING.

Lifeasible provides our clients worldwide with efficient and reliable one-stop services for plant mutation breeding, including mutation induction, mutation detection, and validation of candidate gene function. Devoted to highest-level customer satisfaction, our excellent scientists, experts and technical support teams will work closely with our customers, making sure each project is given the attention and support it needs. Welcome to check our product catalogs and services for details; and please feel free to contact us for questions, inquiries, or collaborations.


Figure 1. Construction of TILLING population in rice and identification of mutants (Koh et al., 2015)

Reference:

  1. Koh, H. J., Kwon, S. Y., and Thomson, M. (2015). Current technologies in plant molecular breeding. Springer Dordrecht Heidelberg, New York, London.
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