Lifeasible has decades of experience in plant genetic engineering. Although the global crop yield has been improved over the recent years, The growth of crops in many areas are still stressed from plant diseases, pests and multiple abiotic stress such as salt, drought, coldness and heavy metal pollution. Plant genetic engineering, also known as plant genetic modification or manipulation, is the key that opens up the doors for introducing crops with valuable traits to produce plants that require fewer pesticides, fungicides, or fertilizers, and can be more resistant to stress conditions. Plant genetic engineering techniques allow direct transfer of one or just a few genes of interest, between either closely or distantly related species to obtain the desired agronomic traits. Aside from adopting genes from other species, plants can also be modified by knock-out, knock-down, or overexpression of their own genes.
Lifeasible addresses the genetic modification of plants through multiple popular genetic engineering technologies, including CRISPR/CAS9, CRISPR base editors, transcription activator-like effector nucleases (TALENs), zinc finger nucleases (ZFNs), RNA interference (RNAi), virus-induced gene silencing (VIGS), and gene overexpression. These technologies play essential roles in modern agriculture for crop optimization. With years of experience in crop breeding and the most advanced plant molecular biology technologies, Lifeasible is determined to provide the professional and customized one-stop services in plant genetic engineering. We proudly provide a full array of services including gene cloning, vector constructions, plasmid transformation, and subsequent phenotype and gene function analysis.
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR Associated Protein9) is a technique that promises rapid editing of genomic DNA with high efficiency and specificity. Utilizing the repairing of DNA double strand breaks (DSBs) induced by CAS9 and single-guide RNA (sgRNA), CRISPR/Cas9 allows precise mutation of one specific gene, which has been successfully applied in plant genetic modification.
CRISPR Base Editors are advanced CRISPR/CAS9 systems that introduce single nucleotide change at target loci. Without the need of double stranded break formation or a donor DNA template, CRISPR Base Editors have been applied successfully in monocots and dicots such as wheat, rice, maize, Arabidopsis, and tomato.
Transcription Activator-like Effector Nucleases (TALENs) are highly efficient and versatile tools for genome editing and have been extensively used in plant gene function research as well as plant breeding. The TALENs consist of TALE DNA-binding domains and restriction endonuclease FokI cleavage domains, which are responsible for introducing of DSBs, followed by endogenous DSBs repairation.
Zinc Finger Nucleases (ZFNs), harboring a zinc finger DNA-binding domain and a FokI DNA cleavage domain, are artificial restriction enzymes engineered for genome editing. This technique is regarded as a promising tool for plant genome edition and crop quality improvement.
RNA Interference (RNAi) is a classical approach for generating sequence-specific gene knock-down or knock-out by introducing specific double-stranded RNA (dsRNA) in the plant system. Due to its high efficiency, quickness, and general affordability, RNAi has become a promising approach for high-tech crop improvement.
Virus-induced Gene Silencing (VIGS) is one of the most powerful reverse genetics tools for rapid determination of plant gene functions. This technique utilizes small interfering RNAs (siRNAs) to accomplish mRNA degradation, translational suppression, or transcriptional inhibitions of targeted genes. So far, the VIGS technique has been adopted in multiple monocot and dicot plant species.
Gene Overexpression is an alternative or complementary method to loss-of-function approaches, which helps to elucidate plant gene functions and introduce new plant traits. In general, expressions of desired genes driven by constitutive or tissue-specific promoters are introduced into plant cells and subsequent study of biological functions. Many commercially available vectors containing fluorescent fusion proteins also are used for subcellular tracking of the interest genes.