Genome editing has always been a frontier and hot area of biological research. Genome editing tools based on CRISPR-Cas9 and CRISPR/Cas12a have been widely used in basic research and applications, such as gene function analysis, germplasm creation, gene therapy, etc. Compared with CRISPR-Cas9 and CRISPR/Cas12a systems, CRISPR/Cas12b is a universal and customizable system in plants, which can finally provide effective gene editing to achieve target gene activation and inhibition.
Figure 1. Protein domain structure of Cas12b. (Strecker et al., 2019)
As a dual-RNA-guided nuclease, Cas12b contains a single RuvC domain and requires both CRISPR RNA (crRNA) and transactivating crRNA (tracrRNAs), which can be combined as a single guide RNA (sgRNA), for DNA targeting. As a class 2 type V-B CRISPR system, Cas12b prefers T-rich protospacer adjacent motifs (PAMs) and generates staggered ends of DNA double-strand breaks. CRISPR/Cas12b provides a higher efficiency for gene activation and a wider target site for gene suppression in plant gene editing.
Figure 2. Schematic of Cas12b sgRNA design. (Strecker et al. 2019)
Advantages of the CRISPR/Cas12b System.
Lifeasible is a world-class phytotechnology company with extensive experience in plant gene editing. We are devoted to optimizing the CRISPR system to achieve genetic modification. Lifeasible has developed a number of Cas12b vectors such as Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) and plant transformation systems of tobacco, potato, cotton, pepper, barley, Arabidopsis, soybean, rice, which are readily available to accommodate the diverse needs of the clients.
CRISPR/Cas12b system experiment procedure
We hope that our technology and services can help you achieve new breakthroughs. For more information, please contact Lifeasible.
References
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following: