Fungi are a large group of microorganisms and many cellular organisms. Fungi are divided into two major groups: unicellular fungi, which are yeasts, and multicellular fungi, which are molds or filamentous fungi. Isolation and identification of fungi is the isolation and culture of fungi and then, according to the characteristics of the colony and microscopic morphology, structure to determine the species. If necessary, through biochemical reactions, identification tests, animal inoculation, and other methods to clarify the strain. There are four types of genes encoding ribosomal RNA in the fungal genome, namely 28S rDNA, 5S rDNA, 18S rDNA, and 5.8S rDNA. 28S rDNA on the large subunit of fungi can be divided into several regions, such as D1 and D2, of which the D1/D2 region is about 500 bp in length and can be used for the identification of fungi. The fungal internal transcribed space (ITS) genes, including ITS1 and ITS2, are fast evolving, polymorphic, and suitable for identifying closely related strains.
Lifeasible can provide various tools to help customers identify fungal species, including morphological observation, physiological and biochemical identification, and molecular biology identification.
Sequence analysis of fungal 28S rDNA D1/D2 region.
PCR amplification of the fungal 28S rDNA D1/D2 region fragment, which is around 500-600bp in length, requires only one sequencing reaction to obtain the 28S rDNA D1/D2 region sequence information. This method is particularly suitable for the identification of yeasts.
Full sequence analysis of fungal ITS region.
PCR amplifies fragments of fungal ITS region with a length of about 300-1,000 bp. Based on the length of PCR amplified fragments, one or two sequencing reactions are performed to obtain the ITS region sequence information.
Lifeasible can help customers isolate and identify fungi based on morphology, molecular biology, and other techniques. As your trusted partner, we can meet your needs for mycorrhizal fungi identification and isolation services and provide efficient and high-quality services. If you want to know the details, please contact us.
Reference
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