Detection of Superoxide Dismutase in Plant Cells

Superoxide dismutase (SOD) is a metal-containing enzyme commonly found in aerobic organisms. Oxygen radicals can be disproportionated by SOD, producing hydrogen peroxide. Catalase can then convert hydrogen peroxide into water and safe molecular oxygen. Hydrogen peroxide is created when SOD catalyzes the disproportionation of oxygen radicals. Catalase then converts hydrogen peroxide into water and safe molecular oxygen.

Lifeasible is a multidisciplinary plant research organization that caters to the demands of agriculture. We offer researchers top-notch physicochemical analysis and cellular composition testing services for plant cell oxidative composition identification based on our cutting-edge tools and experimental platforms. Our plant cell SOD test service can precisely measure the SOD concentration in tissues, which aids in revealing the physiological status of plant cells and plays a significant role in studies on stress resistance, senescence in plant cells, and other related topics.

Physiological role and application pathway of SOD

When plants are stressed by external physiological or non-physiological factors and produce excessive reactive oxygen species, plant superoxide dismutase can scavenge these oxygen radicals, thus preventing oxygen radicals from damaging the composition, structure, and function of the cells and protecting the cells from oxidative damage. It has been shown that, when subjected to external stress, plants in different environmental conditions or different stages of growth and development, and even different tissues and organs in their SOD activity performance are significantly different. The ability to tolerate external stress, that is, resistance to strong plants compared with resistance to weak plants, its body SOD activity is significantly higher, so the expression of the SOD gene is also related to plant stress tolerance.

There is also an essential link between SOD and plant senescence. Several studies have shown that oxidative damage to the plant organism causing senescence and death is due to the production of large amounts of reactive oxygen species from damage to the plant. For example, through the observation of SOD activity changes in rice leaves from the grouting stage to the maturity stage, we can see that the aging process of rice leaves is the decline of SOD activity, the accumulation of reactive oxygen species, and the process of membrane lipid peroxidation is constantly strengthened.

Determination of SOD in plant cells

Superoxide dismutase (SOD) is an essential member of the antioxidant enzyme family and antioxidant metalloenzymes widely distributed in plants, which catalyzes the disproportionation of superoxide anion radicals to produce oxygen and hydrogen peroxide and plays a crucial role in the oxidative and antioxidant homeostasis of the organism. We provide the main assays for superoxide dismutase activity, such as the direct method, catechol autoxidation method, cytochrome C reduction method, chemiluminescence method, and fluorescence kinetic method. In addition, we have established many new methods, such as immunology, simple gel filtration diffusion, polarographic oxygen electrode, microactivity measurement, and many other assays. We can provide customized solutions according to customers' experimental requirements and select the most suitable assay to help customers detect changes in superoxide dismutase activity efficiently and accurately. In addition, we also provide other oxidative stress assay services to meet your different needs.

What do we offer?

At the end of the project, we will provide a detailed technical report, which includes as follows:

• Experimental procedure.
• Relevant parameters.
• Pictures.
• Raw data.
• Superoxide dismutase content/activity information.

Our services workflow

Lifeasible is dedicated to plant life science research and aims to provide comprehensive plant research services. We have rich research and service experience in plant cell biology research. Based on our advanced technology platform and professional research team, we aim to provide high-quality technical services to our clients. Please feel free to contact us for more information.