Bioinformatics Analysis of Plant Cytochrome P450

Cytochrome P450 is a group B cytochrome superfamily protease with heme as a cofactor and is an important heme protein in plant metabolism. Cytochrome P45 has a variety of complex catalytic functions and is involved in the synthesis and metabolism of terpenoids, alkaloids, sterols, fatty acids, and phytohormones in plants. In recent years, the use of bioinformatics tools to study Cytochrome P450 in depth has become a hot research topic.

Lifeasible has an established sequencing laboratory, and data analysis experts focused on providing high-quality cytochrome P450 bioinformatics analysis services. Our customizable analysis services can help clients to elucidate the protein properties, conserved structural domains, and metabolic mechanisms of Cytochrome P450 in plants of interest.

What We Offer

Cytochrome P450 is involved in catalyzing a variety of primary and secondary metabolic reactions in plants. We can perform sequencing services and complete bioinformatics analysis, including but not limited to physicochemical properties, protein signal peptides, protein secondary structure, conserved structural domains, and evolutionary relationships, for various plants, including cabbage, barley, Arabidopsis, and tobacco.

• Physical and Chemical Characterization

We used the ProtParam online tool to analyze the amino acid sequences obtained and can obtain sequence lengths, amino acid numbers, and pI values for the cytochrome P450 of the sample plants.

• ORF Predictive Analysis

We will choose the most suitable sequencing method according to different types of samples, remove the adapter sequence from the obtained data, and splice to get clean data. Then use the ORF finder online tool to predict the ORF of different plant cytochrome P450 nucleic acid sequences.

• Analysis of Signal Peptides of Cytochrome P450

We usually use Signal P 4.1 Server to analyze the signal peptide in the cytochrome P450 sequence of the plant to be tested. Not all plants have signal peptides, but plants that detect signal peptides can infer the main types of proteins produced, where they are transported, and the reactions they may be involved in.

• Cytochrome P450 Phylogenetic Tree Construction

We will use MEGA 11.0 to construct a phylogenetic tree. Four different algorithms (Maximum Likelihood, Neighbor-joining, Mini-mum Evolution Tree, and Maximum Parsimony) will be used to compare the constructed evolutionary trees and further clarify the evolutionary relationships between plants.

Applications

• To elucidate the taxonomic and evolutionary relationships of plants.
• Use Cytochrome P450 fermentation to produce valuable chemicals.
• Explore the properties of Cytochrome P450 to develop novel enzymes to catalyze specific chemosynthetic reactions.

Why Choose Lifeasible

We have complete laboratory facilities and a strict quality management system. We offer optimized protocols and a wide range of bioinformatics tools for different samples to meet all our client's requirements, saving them time and costs.

How to Place an Order

Lifeasible has been dedicated to providing customizable bioinformatics analysis of plant cytochrome P450 for many years. Please feel free to contact us with your questions, needs, or collaborations.