Product Name
                            
                            
                                c-Myc tag, HRP conjugated (clone 9E10)                            
                         
                                                
                                                
                                                
                            
                                Immunogen
                            
                            
                                Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106                            
                         
                                                
                                                
                            
                                Background
                            
                            
                                c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.                            
                         
                                                
                            
                                Format
                            
                            
                                Liquid at 1 mg/ml.                            
                         
                                                
                                                
                            
                                Purity
                            
                            
                                Conjugated with horseradish peroxidase (HRP) of high specific activity.                            
                         
                                                
                                                
                            
                                Reactivity
                            
                            
                                c-myc tagged proteins                            
                         
                                                
                            
                                Confirmed reactivity
                            
                            
                                c-Myc tagged fusion proteins                            
                         
                                                
                            
                                Tested applications
                            
                            
                                Western blot (WB)                            
                         
                                                
                            
                                Recommended dilution
                            
                            
                                1-2 µg/ml (WB)                            
                         
                                                
                            
                                Storage
                            
                            
                                Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.