3' Primer
                            
                            
                                GFP-R primer: ACCATCTAATTCAACAAGAATTGGGACAAC                            
                         
                                                
                            
                                5' Primer
                            
                            
                                M13/pUC fwd primer: CCAGTCACGACGTTGTAAAACG                            
                         
                                                
                            
                                Cloning Method
                            
                            
                                MCS, Restriction endonucleases                            
                         
                                                
                            
                                Cloned Strain(s)
                            
                            
                                DH10B competent cells, 30°C                            
                         
                                                
                            
                                Host cells (lines)
                            
                            
                                Sprouting yeast (Saccharomyces cerevisiae), Candida albicans                            
                         
                                                
                            
                                Stable
                            
                            
                                Transient expression                            
                         
                                                
                            
                                Selectable Marker
                            
                            
                                URA3, Nourseothricin                            
                         
                                                
                            
                                Constitutive/Inducible
                            
                            
                                Constitutive                            
                         
                                                
                            
                                Bacterial Resistance(s)
                            
                            
                                Ampicillin                            
                         
                                                
                                                
                                                
                            
                                Plasmid Type
                            
                            
                                Saccharomyces cerevisiae expression vectors, Integrated vectors, FLP/FRT system vectors