3' Primer
                            
                            
                                AUG1-R: GAAGAGAAAAACATTAGTTGGC                            
                         
                                                
                            
                                5' Primer
                            
                            
                                AUG1-F: CAATTTACATCTTTATTTATTAACG                            
                         
                                                
                            
                                Cloning Method
                            
                            
                                MCS, Restriction endonucleases                            
                         
                                                
                                                
                                                
                            
                                Constitutive/Inducible
                            
                            
                                Inducible                            
                         
                                                
                            
                                Induction Method
                            
                            
                                Methanol                            
                         
                                                
                            
                                Bacterial Resistance(s)
                            
                            
                                Ampicillin                            
                         
                                                
                                                
                                                
                            
                                Plasmid Type
                            
                            
                                Pichia pastor protein expression vectors