Product Name
Hemin Colorimetric Assay Kit
Product Overview
Heme is a complex molecule with an iron atom at its center, surrounded by a porphyrin ring. This structure enables heme to bind with oxygen and other molecules, making it essential for oxygen transport and carbon dioxide removal in the blood. In medical examinations, heme is crucial for several tests. Measuring hemoglobin levels assesses the blood's oxygen-carrying capacity. The presence of heme in urine can indicate conditions such as hemolytic anemia, renal diseases, and certain cancers.
Description
Horseradish Peroxidase (HRP) is a glycoprotein enzyme extracted from the roots of the horseradish plant (Armoracia rusticana). It contains a cofactor structure similar to that of heme, the absence of which leads to the formation of Apo-HRP. When it attaches to a Heme group, it is able to catalyze the oxidation reaction by H2O2. In this assay, HRP-heme will catalyze a oxidation reaction, which transform a color agent (Reagent2) into a blueish complex. It can be detected by spectrophotometer at 670nm, which can be used to calculate the concentration of Heme groups in the sample.
Applications
Direct Assays:Total heme in blood, serum, plasma, urine, heme-carrying enzymes.
Pharmacology: Effects of drugs on heme metabolism.
Drug Discovery: HTS for drugs that modulate heme levels.
Kit Components
1. Bottle1 Reagent1(Apo-HRP plus Buffer): 40ml, pH=6.5±0.1. Stable for> 1 year at 4°C.
2. Bottle2 Reagent2(color agent): 10ml, pH=3.33. Stable for> 1 year at 4°C.
3.Bottle3 Calibrator(equivalent to 62.5 µM heme): 2ml. Stable for> 1 year at 4°C.
Materials Required but Not Supplied
Pipeting devices and accessories.
Datasheet 