L-Serine (with 340nm) Assay Kit

L-Serine (with 340nm) Assay Kit

AgK-410

L-Serine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. L-Serine (L-Ser) is a non-essential amino acid In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin,and the amino acids glycine and cysteine.L-Ser is importantfor brain development and functions which areespecially sustained by altered L-Ser levels inpsychiatric disorders and occurrence of severeneurological abnormalities in patients withdefects in L-Ser biosynthesis.

L-homocysteine (HCY) is converted to cystathionine by the use of CBS (cystathionine beta-synthase) and L-Serine.The cystathionine is then broken down to homocysteine, pyruvate and ammonia. Pyruvate is converted to lactate via lactate dehydrogenase with NADH as coenzyme. The rate of NADH conversion to NAD+ (Δ A340 nm) is directly proportional to the concentration of L-Serine.

Estimation of Serine in various biological samples

100 assays

1.Tris Buffer, LDH - 35 KU/L, TCEP - 0.5 mmol/L, NADH - 0.47 mmol/L, PRESERVATIVES

2.Tris Buffer, Cystathionine β-Synthase - 20 KU/L, Cystathionine β-lyase - 10 KU/L, PRESERVATIVES

3.L-homocysteine

4.Dilution buffer

5.L-serine cal (liquid)

6.L-serine control (liquid)

Clinical chemistry analysers

340 nm