Phomopsis viticola Real-Time PCR Control (100)

Phomopsis viticola Real-Time PCR Control (100)

Cat#
CPD-578
Product Name
Phomopsis viticola Real-Time PCR Control (100)
Product Overview
Phomopsis viticola Real-Time PCR Control (100) is designed for the detection of Phomopsis viticola DNA based on the use of real-time PCR technology.
Description
Phomopsis viticola Real-Time PCR Control (100) is designed for the detection of Phomopsis viticola specific DNA based on the use of real-time PCR technology. The detection of Phomopsis viticola specific DNA is providing a simple, reliable and rapid result for the detection of Phomopsis viticola infection. Phomopsis viticola Real-Time PCR Control (100) includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Phomopsis viticola Real-Time PCR Control (100) comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.
Storage
All kit components should be stored at -20°C upon arrival;
Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots;
All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Kit Components
Component Product
2X PCR Master Mix 350 μL
Phomopsis viticola Primer & Probe Mix 70 μL
Phomopsis viticola Positive Control 50 μL
Nuclease-Free Water (Negative control) 1.25 mL
Product Insert 1
Materials Required but Not Supplied
Appropriate Real-Time PCR Instrument with FAM and HEX filter channel;
DNA Purification Kit: The kit is compatible with all DNA purification kits that yield high quality, inhibitor-free DNA;
Disposable powder-free gloves;
Benchtop microcentrifuge;
Micropipettors;
Sterile pipette tips with filters;
PCR tubes;
Vortex mixer;
PCR reaction preparation station (Optional).
Scientific Background
Phomopsis cane and leaf spot, caused by Phomopsis viticola, occurs in most grape-growing regions and is an important disease of grapes worldwide. The fungus attacks all green parts of the vine, including canes, leaves, flowers, rachises, and berries. However, most economic damage is caused by rachis and berry infection. Infected leaves have small, yellowish spots with dark brown centers and may be puckered. With successive cool, wet springs, the inoculum will build up and infections will become more severe over time. Since symptoms appear 21 to 30 days after infection, early detection of the pathogen in the vineyard is therefore crucial for its control.
Detection method
Real-Time PCR
Sample Type
Plant tissues
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