Proteins, as the main executors of life activities, have far-reaching impacts on crop growth, development, and stress resistance through their expression, modification, and functional changes. Targeted proteomics, as an advanced technological tool, can accurately quantify and analyze specific target proteins in complex biological samples, providing strong support for agricultural scientific research.

Lifeasible provides targeted proteomics analysis services using a high-throughput mass spectrometry platform, aiming to quantify the characteristic proteins more accurately and sensitively.

Explore Our Targeted Proteomics Analysis

Our targeted proteomics strategy, relying on PRM technology, provides more specific and sensitive quantitative analysis of target proteins in complex biological samples. Our targeted proteomic PRM (Parallel Reaction Monitoring) is a targeted quantification technology based on high-resolution, high-precision mass spectrometry that enables selective detection of target proteins for precise quantification.

Advantages of PRM

• High mass accuracy can better eliminate background interference and false positive problems and effectively improve the detection limit and sensitivity under complex background.
• Full scanning of product ions, no need to select ion pairs, easier to establish analytical methods.
• Wide linear range.

Relying on our advanced target-image proteomics technology platform, our targeted proteomics services cover the following core programs.

• Quantitative analysis of target proteins. Utilizing high-precision mass spectrometry technology, we can accurately quantify target proteins in agricultural biological samples and provide accurate protein expression level data.
• Protein post-translational modification studies. For common post-translational modifications such as phosphorylation, glycosylation, ubiquitination, etc., we provide professional modification site identification and quantification services to help reveal key biological processes such as protein activity regulation and cell signal transduction.
• Biomarker validation. Assist in the screening and validating biomarkers closely related to agricultural traits to accelerate the process of crop improvement, disease early warning, and other applications.

Application of Targeted Proteomics Analysis

• Crop quality research. In-depth analysis of quality-related proteins in crop grains, leaves, and other tissues, revealing the molecular mechanism of their synthesis and accumulation, and providing a theoretical basis for cultivating high-yield and high-quality crop varieties.
• Research on the mechanism of resistance to pests and diseases. To study the changes of resistance-related proteins in plants when they are attacked by pathogenic bacteria and pests, and to clarify the mechanism of resistance, which will help to develop new anti-pest and disease resistance strategies and cultivate resistant varieties.
• Plant hormone signaling research. Targeted detection of plant hormone receptors, signaling proteins, etc., to analyze the hormone regulatory network, to provide targets for regulating plant growth and development, and to improve crop adaptation.
• Environmental stress response research. Analyze the expression changes of anti-stress-related proteins under plant adversity conditions, explore key anti-stress genes and proteins, and support the cultivation of anti-stress crops.

Fig.2 Process of our service. (Lifeasible)

Highlights of Our Targeted Proteomics Analysis

• Cutting-edge mass spectrometry equipment. We are equipped with state-of-the-art mass spectrometers to support high-resolution, high-coverage targeting analysis.
• Customized sample pre-treatment. We optimize lysis and enrichment protocols for complex agricultural samples to improve membrane or low-abundance proteins' detection rate.
• Full-flow quality control system. We follow relevant standards and set up strict quality control points from sample preparation to data analysis to ensure reproducible results across batches.

Lifeasible ensures high-quality services in its proteomics analysis services, and through our targeted proteomics analysis services, we can help you gain a deeper understanding of protein research. If you are interested, please feel free to contact us.